super(31)P NMR study of daunorubicin-d(CGTACG) complex in solution. Evidence of the intercalation sites

The interaction of daunorubicin with the self-complementary DNA fragment d(CGTACG) was studied by super(31)P NMR spectroscopy. The individual phosphates have been assigned for the nucleotide and the complex and signals from bound and free species in slow exchange at 19 degree C were detected. In sol...

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Bibliographic Details
Published in:FEBS letters 1988-01, Vol.236 (1), p.231-234
Main Authors: Ragg, E, Mondelli, R, Battistini, C, Garbesi, A, Colonna, F P
Format: Article
Language:English
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Summary:The interaction of daunorubicin with the self-complementary DNA fragment d(CGTACG) was studied by super(31)P NMR spectroscopy. The individual phosphates have been assigned for the nucleotide and the complex and signals from bound and free species in slow exchange at 19 degree C were detected. In solution, the hexanucleotide binds two molecules of daunorubicin, which intercalate in the d(CG) sequence at both ends of the helix. Evidence for local deformations of the backbone at the sites of C sub(5)pG sub(6), C sub(1)pG sub(2) and G sub(2)pT sub(3) phosphates is given. The binding constants for the stepwise equilibrium and the rate of dissociation of the intercalated duplex were also determined.
ISSN:0014-5793