O super(6)-Ethylguanine carcinogenic lesions in DNA: An NMR study of O super(6)etG multiplied by T pairing in dodecanucleotide duplexes

High-resolution two-dimensional NMR studies are reported on the self-complementary duplex (designated O super(6)-etG multiplied by T 12-mer) containing two symmetrically related O super(6)etG multiplied by T lesion sites located four base pairs in from either end of the duplex. Parallel studies were...

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Bibliographic Details
Published in:Biochemistry (Easton) 1989-01, Vol.28 (15), p.6170-6181
Main Authors: Kalnik, M W, Li, BFL, Swann, P F, Patel, D J
Format: Article
Language:English
Online Access:Get full text
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Summary:High-resolution two-dimensional NMR studies are reported on the self-complementary duplex (designated O super(6)-etG multiplied by T 12-mer) containing two symmetrically related O super(6)etG multiplied by T lesion sites located four base pairs in from either end of the duplex. Parallel studies were undertaken on a related sequence containing O super(6)meG multiplied by T lesion sites (designated O super(6)meG multiplied by T 12-mer) in order to evaluate the influence of the size of the alkyl substituent on the structure of the duplex and were undertaken on a related sequence containing G multiplied by T mismatch sites (designated G multiplied by T 12-mer duplex), which served as the control duplex. The exchangeable and nonexchangeable proton and the phosphorus nuclei have been assigned from an analysis of two-dimensional nuclear Overhauser enhancement (NOE) and correlated spectra of the O super(6)etG multiplied by T 12-mer, O super(6)-meG multiplied by T 12-mer, and G multiplied by T 12-mer duplexes in H sub(2)O and D sub(2)O solutions.
ISSN:0006-2960