Induction of DNA strand breaks and DNA—protein cross-links in normal human skin fibroblasts following exposure to 254 nm UV radiation

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 1990-08, Vol.6 (4), p.395-404
• Main Authors: Lai, Li-Wen, Rosenstein, Barry S.
• Format: Article
• Language: English
• Subjects: 254 nm UV; Biological and medical sciences; Biological effects of radiation; Cell Line; Cross-Linking Reagents; DNA - metabolism; DNA - radiation effects; DNA Damage; DNA strand breakage; DNA—protein cross-linking; Dose-Response Relationship, Radiation; Fibroblasts - drug effects; Fundamental and applied biological sciences. Psychology; human skin cells; Humans; Kinetics; Non ionizing radiations. Hertzian waves. Biooptics; Proteins - metabolism; Proteins - radiation effects; Skin - radiation effects; Tissues, organs and organisms biophysics; Ultraviolet Rays
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/1011-1344(90)85113-B

Levels of DNA strand breaks and DNA—protein cross-links (DPCs) were measured using the alkaline elution assay in normal human skin fibroblasts irradiated with 0–200 J m −2 of 254 nm UV radiation and incubated for 0–24 h. On incubation, the yields of both single-strand breaks (SSBs) and DPCs increased with similar kinetics and remained elevated. In addition, when SSBs were measured under conditions in which DPCs were not eliminated by treatment with proteinase K, a measurable yield of SSBs could not be detected. Hence, the SSBs that form in the UV-irradiated cells following incubation appear to be associated with the DPCs.