Influence of phospholipid composition on antibody responses to liposome encapsulated protein and peptide antigens

• Published in: Vaccine 1996-06, Vol.14 (9), p.898-904
• Main Authors: Phillips, Nigel C., Gagné, Lucie, Ivanoff, Nathalie, Riveau, Gilles
• Format: Article
• Language: English
• Subjects: Adjuvant; Adjuvants, Immunologic - chemistry; alum; Amino Acid Sequence; Animals; Antibody Formation - immunology; Antigens - immunology; Applied microbiology; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; IgA; IgE; IgG; IgG 2a; IgG 2b; Immunoglobulin A - biosynthesis; Immunoglobulin E - biosynthesis; Immunoglobulin G - biosynthesis; liposome; Liposomes - chemistry; Liposomes - immunology; Liposomes - toxicity; Macrophages - drug effects; MDP; Mice; Mice, Inbred Strains; Microbiology; Molecular Sequence Data; Peptides - immunology; Phospholipids - analysis; Phospholipids - immunology; Proteins - immunology; Serum Albumin, Bovine - immunology; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); zeta potential
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/0264-410X(96)82949-5

The effect of phospholipid composition on mouse IgG antibody responses to liposomal bovine serum albumin (BSA), murine monoclonal antibody GK1.5 (anti-CD4) or a 21 amino acid peptide from the second conserved domain of HIV gp120 after s.c. administration, and on the IgA, IgE, and IgG antibody response to liposomal Shistosoma mansoni glutathione- S-transferase (Sm28GST) after oral administration, was determined. Antibody responses were compared with alum-adsorbed and N-acetylmuramyl-L-alanyl-D-isoglutamine (MDP)-antigen mixtures. For the s.c. route, dipalmitoylphosphatidylcholine (DPPC)/dimyristoylphosphatidylglycerol (DMPG) liposomes induced 54–60% IgG 1 and 35–44% IgG 2a+2b. DPPC/dipalmitoylphosphatidylethanolamine (DPPE) liposomes induced 73–78% IgG 1 and 15–25% IgG 2a+2b. DPPC/phosphatidylserine (PS) liposomes induced 86–89% IgG 1 and 8–12% IgG 2a+2b. Alum and MDP induced 79–91% IgG 1 and 4–17% IgG 2a+2b. The rank order of adjuvanticity for induction of IgG antibody was DPPC/DMPGDPPC/PE ⪢ alum ⪢ MDPDPPC/PS for all three antigens. DPPC/DMPG liposomes were the only effective adjuvant for the induction of secretory IgA and circulatory IgE and IgG antibodies against Sm28GST after oral adminstration. The failure of liposome-antigen mixtures to elicit an antibody response showed that liposomal incorporation of the antigens was obligatory for adjuvant activity. These results demonstrate that the correlation between phospholipid composition and adjuvanticity is independent of liposome charge, antigen, or route of administration.