Production of non-chimeral colchiploids in Rubus species by tissue culture

Diploid Rubus allegheniensis Porter and R. rusticanus Merc. shoot tips were proliferated in tissue culture and treated with 0.02 to 0.08% colchicine to induce non-chimeral polyploidy. About 20 shoot tips of each treatment were transferred to test tubes containing modified Anderson's medium for...

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Bibliographic Details
Published in:Euphytica 1989-11, Vol.44 (1), p.133-135
Main Author: Gupton, C.L. (Small Fruit Research Station USDA-ARS, Poplarville, MS (USA))
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
COLCHICINA
COLCHICINE
CULTIVO DE TEJIDOS
CULTURE DE TISSUS
Economic plant physiology
ESTOMA
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
GROSSEUR
In vitro culture
POLEN
POLLEN
Pteridophyta, spermatophyta
RUBUS
RUBUS ALLEGHENIENSIS
SIZE
STOMATA
STOMATE
TAMANO
TETRAPLOIDE
TETRAPLOIDS
TISSUE CULTURE
Vegetals
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Summary:Diploid Rubus allegheniensis Porter and R. rusticanus Merc. shoot tips were proliferated in tissue culture and treated with 0.02 to 0.08% colchicine to induce non-chimeral polyploidy. About 20 shoot tips of each treatment were transferred to test tubes containing modified Anderson's medium for shoot proliferation. Shoots at the 3- or 4-leaf stage were transplanted into Jiffy-7 peat pellets and placed under intermittent mist for rooting. Root tip squashes were made for chromosome counts. Thirteen R. allegheniensis and 7 R. rusticanus plants proved to be autotetraploids (28 chromosomes).
ISSN:0014-2336
1573-5060
DOI:10.1007/BF00022607