The rapid analysis of neutral and polar marine lipids using double-development HPTLC and scanning densitometry

A method is described for the separation by high-performance thin-layer chromatography (HPTLC) of marine lipids on one plate using two developments in succession, one for polar lipids and one for neutral lipids. 14 lipid classes can be separated by the solvent system employed. Quantitation of the se...

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Bibliographic Details
Published in:Journal of experimental marine biology and ecology 1989-01, Vol.129 (2), p.189-197
Main Authors: Olsen, R.E., Henderson, R.J.
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Biological and medical sciences
Double development
Fundamental and applied biological sciences. Psychology
General aspects. Techniques
HPTLC
Marine
Marine lipid
Method
Methods and techniques (sampling, tagging, trapping, modelling...)
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Summary:A method is described for the separation by high-performance thin-layer chromatography (HPTLC) of marine lipids on one plate using two developments in succession, one for polar lipids and one for neutral lipids. 14 lipid classes can be separated by the solvent system employed. Quantitation of the separated lipid classes can be achieved using scanning densitiometry. The method can be applied to lipids extracted from marine organisms including the complex polar lipids derived from phytoplankton and fish neural tissue.
ISSN:0022-0981
1879-1697
DOI:10.1016/0022-0981(89)90056-7