Production of extracellular enzymes by immobilized Trichoderma reesei in shake flask cultures

• Published in: Applied microbiology and biotechnology 1995-01, Vol.43 (5), p.815-821
• Main Authors: Haapala, R, Parkkinen, E, Suominen, P, Linko, S
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; celluloses; Enzyme engineering; Fermentation; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Production of selected enzymes; Trichoderma reesei; xylanases
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/BF02431913

Polyurethane foam and nylon-web carriers were compared for simultaneous production of endo-1,4-beta-glucanase and xylanase by immobilized Trichoderma reesei on a medium based on lactose (27 g/l), cellulose (3 g/l) and sorbose (0.3 g/l). Nylon sheet with 1.2 cm2 carrier surface/ml medium was superior to the others and it was selected for further studies. The carbon source had a marked effect on enzyme production by the immobilized fungus. With pure cellulose (10 g/l) as substrate, the maximum endoglucanase activity was 690 nkat/ml and xylanase activity 4800 nkat/ml. Supplementation with 0.5 g/l of sorbose resulted in an increase in both endoglucanase and xylanase activities in all media studied. A more detailed study on the effect of sorbose on a lactose(7 g/l)- and cellulose(3 g/l)-based medium revealed a clear optimum sorbose concentration of 1.5 g/l with a maximum endoglucanase activity of 660 nkat/ml, xylanase activity of 3670 nkat/ml, and filter-paper activity (overall cellulolytic activity) of 2.0 filter-paper units/ml. However, the addition of 1.5 g/l sorbose to the pure-cellulose(10 g/l)-based medium resulted in a slight decrease in the enzyme production.