Fermentation broth clarification techniques

This review describes the two main separation processes currently used to recover cultured cells from fermentation broth at process scale; centrifugation and microfiltration. The fundamental principles of each technique are presented and the significant parameters that affect process efficiency are...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1990-02, Vol.23 (2), p.91-121
Main Author: Whittington, P.N
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
cell suspension culture
centrifugation
clarification
fermentation
filtration
flocculation
Fundamental and applied biological sciences. Psychology
laboratory techniques
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
microfiltration
separation
separation techniques
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Summary:This review describes the two main separation processes currently used to recover cultured cells from fermentation broth at process scale; centrifugation and microfiltration. The fundamental principles of each technique are presented and the significant parameters that affect process efficiency are highlighted. Strategies to optimize these parameters and hence process efficiency are suggested with reference to the properties of cells and fermentation broth. The process enabling technology of flocculation is also reviewed, since it is possible to enhance centrifugal separation efficiency using this technique.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02798381