Comparison of Vero cell assay and PCR as indicators of the presence of verocytotoxigenic Escherichia coli in bovine and human fecal samples

Comparisons were made between Vero cell assay (VCA) and PCR as indicators for the detection of verocytotoxigenic Escherichia coli (VTEC; also known as Shiga-like toxin-producing E. coli) and as predictors of VTEC isolation from bovine and human fecal samples. Fecal samples were collected as part of...

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Published in:Applied and Environmental Microbiology 1996-12, Vol.62 (12), p.4314-4317
Main Authors: Rahn, K. (Agriculture and Agri-Food Canada, Guelph, Ontario, Canada.), Wilson, J.B, McFadden, K.A, Read, S.C, Ellis, A.G, Renwick, S.A, Clarke, R.C, Johnson, R.P
Format: Article
Language:English
Subjects:
Animals
Bacteria
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
BOVIN LAITIER
Cattle
Cells
Cercopithecus aethiops
CULTIVO DE CELULAS
CULTURE DE CELLULE
DOSAGE BIOLOGIQUE
ENSAYO BIOLOGICO
ESCHERICHIA COLI
Escherichia coli - isolation & purification
Escherichia coli - pathogenicity
ESTIERCOL
EXPLOITATION AGRICOLE FAMILIALE
EXPLOITATION LAITIERE
EXPLOTACION AGRICOLA FAMILIAR
FAMILIA
FAMILLE
FECES
Feces - microbiology
FUMIER
Fundamental and applied biological sciences. Psychology
GANADO DE LECHE
GENERO HUMANO
GENRE HUMAIN
GRANJAS LECHERAS
HECES
HOGARES
Humans
MENAGE
Microbiology
MONO
ONTARIO
PCR
Polymerase Chain Reaction
REIN
RINONES
SINGE
TOXICIDAD
TOXICITE
TOXINAS
TOXINAS BACTERIANAS
TOXINE
TOXINE BACTERIENNE
Vero Cells
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creator Rahn, K. (Agriculture and Agri-Food Canada, Guelph, Ontario, Canada.)
Wilson, J.B
McFadden, K.A
Read, S.C
Ellis, A.G
Renwick, S.A
Clarke, R.C
Johnson, R.P
description Comparisons were made between Vero cell assay (VCA) and PCR as indicators for the detection of verocytotoxigenic Escherichia coli (VTEC; also known as Shiga-like toxin-producing E. coli) and as predictors of VTEC isolation from bovine and human fecal samples. Fecal samples were collected as part of a survey on the prevalence of VTEC on dairy farms in southern Ontario (J. B. Wilson et al., J. Infect. Dis., 174:1021-1027, 1996). A total of 2,655 samples were examined by VCA and PCR, 2,153 originating from cattle and 502 originating from humans. Overall, 36.2% of the samples were positive in the VCA and 38.7% were positive by PCR. Of the VCA-positive samples screened, 41.6% yielded a VTEC isolate. For both human and bovine samples, a significant positive association between PCR result and VCA titer (P = 0.0001) was found. In addition, there was a significant positive association between the PCR result and VTEC isolation from VCA-positive samples for cattle (odds ratio = 9.1, P 0.0001). For bovine samples positive in the VCA, VCA titer was significantly associated with the probability of obtaining a VTEC isolate. Agreement between VCA and PCR was good for both bovine and human samples (kappa
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(Agriculture and Agri-Food Canada, Guelph, Ontario, Canada.) ; Wilson, J.B ; McFadden, K.A ; Read, S.C ; Ellis, A.G ; Renwick, S.A ; Clarke, R.C ; Johnson, R.P</creator><creatorcontrib>Rahn, K. (Agriculture and Agri-Food Canada, Guelph, Ontario, Canada.) ; Wilson, J.B ; McFadden, K.A ; Read, S.C ; Ellis, A.G ; Renwick, S.A ; Clarke, R.C ; Johnson, R.P</creatorcontrib><description>Comparisons were made between Vero cell assay (VCA) and PCR as indicators for the detection of verocytotoxigenic Escherichia coli (VTEC; also known as Shiga-like toxin-producing E. coli) and as predictors of VTEC isolation from bovine and human fecal samples. Fecal samples were collected as part of a survey on the prevalence of VTEC on dairy farms in southern Ontario (J. B. Wilson et al., J. Infect. Dis., 174:1021-1027, 1996). A total of 2,655 samples were examined by VCA and PCR, 2,153 originating from cattle and 502 originating from humans. Overall, 36.2% of the samples were positive in the VCA and 38.7% were positive by PCR. Of the VCA-positive samples screened, 41.6% yielded a VTEC isolate. For both human and bovine samples, a significant positive association between PCR result and VCA titer (P = 0.0001) was found. In addition, there was a significant positive association between the PCR result and VTEC isolation from VCA-positive samples for cattle (odds ratio = 9.1, P 0.0001). For bovine samples positive in the VCA, VCA titer was significantly associated with the probability of obtaining a VTEC isolate. Agreement between VCA and PCR was good for both bovine and human samples (kappa</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.62.12.4314-4317.1996</identifier><identifier>PMID: 8953703</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacteria ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; BOVIN LAITIER ; Cattle ; Cells ; Cercopithecus aethiops ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; DOSAGE BIOLOGIQUE ; ENSAYO BIOLOGICO ; ESCHERICHIA COLI ; Escherichia coli - isolation &amp; purification ; Escherichia coli - pathogenicity ; ESTIERCOL ; EXPLOITATION AGRICOLE FAMILIALE ; EXPLOITATION LAITIERE ; EXPLOTACION AGRICOLA FAMILIAR ; FAMILIA ; FAMILLE ; FECES ; Feces - microbiology ; FUMIER ; Fundamental and applied biological sciences. Psychology ; GANADO DE LECHE ; GENERO HUMANO ; GENRE HUMAIN ; GRANJAS LECHERAS ; HECES ; HOGARES ; Humans ; MENAGE ; Microbiology ; MONO ; ONTARIO ; PCR ; Polymerase Chain Reaction ; REIN ; RINONES ; SINGE ; TOXICIDAD ; TOXICITE ; TOXINAS ; TOXINAS BACTERIANAS ; TOXINE ; TOXINE BACTERIENNE ; Vero Cells</subject><ispartof>Applied and Environmental Microbiology, 1996-12, Vol.62 (12), p.4314-4317</ispartof><rights>1997 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Dec 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c638t-674505fc5dd53f80aa66b5a221ce4a6d19a7e1df4a89040795895da8ea7e338b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC168258/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC168258/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3186,3187,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=2516121$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8953703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rahn, K. (Agriculture and Agri-Food Canada, Guelph, Ontario, Canada.)</creatorcontrib><creatorcontrib>Wilson, J.B</creatorcontrib><creatorcontrib>McFadden, K.A</creatorcontrib><creatorcontrib>Read, S.C</creatorcontrib><creatorcontrib>Ellis, A.G</creatorcontrib><creatorcontrib>Renwick, S.A</creatorcontrib><creatorcontrib>Clarke, R.C</creatorcontrib><creatorcontrib>Johnson, R.P</creatorcontrib><title>Comparison of Vero cell assay and PCR as indicators of the presence of verocytotoxigenic Escherichia coli in bovine and human fecal samples</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Comparisons were made between Vero cell assay (VCA) and PCR as indicators for the detection of verocytotoxigenic Escherichia coli (VTEC; also known as Shiga-like toxin-producing E. coli) and as predictors of VTEC isolation from bovine and human fecal samples. 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(Agriculture and Agri-Food Canada, Guelph, Ontario, Canada.) ; Wilson, J.B ; McFadden, K.A ; Read, S.C ; Ellis, A.G ; Renwick, S.A ; Clarke, R.C ; Johnson, R.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c638t-674505fc5dd53f80aa66b5a221ce4a6d19a7e1df4a89040795895da8ea7e338b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>BOVIN LAITIER</topic><topic>Cattle</topic><topic>Cells</topic><topic>Cercopithecus aethiops</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>DOSAGE BIOLOGIQUE</topic><topic>ENSAYO BIOLOGICO</topic><topic>ESCHERICHIA COLI</topic><topic>Escherichia coli - isolation &amp; purification</topic><topic>Escherichia coli - pathogenicity</topic><topic>ESTIERCOL</topic><topic>EXPLOITATION AGRICOLE FAMILIALE</topic><topic>EXPLOITATION LAITIERE</topic><topic>EXPLOTACION AGRICOLA FAMILIAR</topic><topic>FAMILIA</topic><topic>FAMILLE</topic><topic>FECES</topic><topic>Feces - microbiology</topic><topic>FUMIER</topic><topic>Fundamental and applied biological sciences. 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source Open Access: PubMed Central; American Society for Microbiology美国微生物学会期刊
subjects Animals
Bacteria
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
BOVIN LAITIER
Cattle
Cells
Cercopithecus aethiops
CULTIVO DE CELULAS
CULTURE DE CELLULE
DOSAGE BIOLOGIQUE
ENSAYO BIOLOGICO
ESCHERICHIA COLI
Escherichia coli - isolation & purification
Escherichia coli - pathogenicity
ESTIERCOL
EXPLOITATION AGRICOLE FAMILIALE
EXPLOITATION LAITIERE
EXPLOTACION AGRICOLA FAMILIAR
FAMILIA
FAMILLE
FECES
Feces - microbiology
FUMIER
Fundamental and applied biological sciences. Psychology
GANADO DE LECHE
GENERO HUMANO
GENRE HUMAIN
GRANJAS LECHERAS
HECES
HOGARES
Humans
MENAGE
Microbiology
MONO
ONTARIO
PCR
Polymerase Chain Reaction
REIN
RINONES
SINGE
TOXICIDAD
TOXICITE
TOXINAS
TOXINAS BACTERIANAS
TOXINE
TOXINE BACTERIENNE
Vero Cells
title Comparison of Vero cell assay and PCR as indicators of the presence of verocytotoxigenic Escherichia coli in bovine and human fecal samples
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