Osteotropic factor-stimulated synthesis of thrombospondin in rat dental pulp cells

The amount of thrombospondin (TSP) mRNA in confluent clonal rat dental pulp cells was increased by transforming growth factor-β (TGF-β), 1α,25-dihydroxyvitamin D 3 (1,25(OH) 2D 3), and phorbol 12,13-didecanoate (PDD), with maximal levels 6, 36 and 2 h, respectively, after stimulation. These increase...

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Bibliographic Details
Published in:FEBS letters 1996-09, Vol.393 (2), p.193-196
Main Authors: Islam, Md.Rokibul, Ueno, Akemichi, Nishikawa, Hiroyuki, Nagata, Toshihiko, Inoue, Hideo
Format: Article
Language:English
Subjects:
1,25(OH)2D3
1α,25-Dihydroxyvitamin D 3
1α,25-Dihydroxyvitamin D3
4β-phorbol 12,13-didecanoate
Animals
Autoradiography
Calcitriol - pharmacology
Carcinogens - pharmacology
Cell Adhesion
Cell Adhesion Molecules - biosynthesis
Clone Cells
Culture Media
Dental Pulp - metabolism
Dental pulp cell
Eagle's minimum essential medium
EMEM
GAPDH
glyceraldehyde 3-phosphate dehydrogenase
Kinetics
Membrane Glycoproteins - biosynthesis
Membrane Glycoproteins - isolation & purification
Methionine - metabolism
OPN
Osteopontin
PDD
Phorbol ester
Phorbol Esters - pharmacology
PKC
protein kinase C
Rats
RNA, Messenger - metabolism
SDS polyacrylamide gel electrophoresis
SDS-PAGE
Sialoglycoproteins - biosynthesis
Sulfur Radioisotopes
TGF-β
Thrombospondin
Thrombospondins
Time Factors
Transcription, Genetic - drug effects
Transforming Growth Factor beta - pharmacology
Transforming growth factor-β
TSP
VDRE
vitamin D response element
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Summary:The amount of thrombospondin (TSP) mRNA in confluent clonal rat dental pulp cells was increased by transforming growth factor-β (TGF-β), 1α,25-dihydroxyvitamin D 3 (1,25(OH) 2D 3), and phorbol 12,13-didecanoate (PDD), with maximal levels 6, 36 and 2 h, respectively, after stimulation. These increases were accompanied by enhanced syntheses of TSP proteins which were found in the different forms in cell layer/matrix fraction (198 and 165 kDa TSP) and the culture medium (180 kDa TSP). These three factors also raised the mRNA level of osteopontin, which is thought to play an important role in mineralization in dentin and bone. The order of effectiveness of these factors was PDD > TGF-β > 1,25(OH) 2D 3 for all the stimulations described above. These results suggest that the osteotropic factors enhance TSP synthesis at the pretranslational level and that TSP produced by dental pulp cells participates in formation of reparative dentin.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)00882-4