Molecular cloning and characterisation of the mouse preprogalanin gene

Using a probe obtained by PCR amplification from mouse genomic DNA, a genomic clone was isolated covering the entire mouse preprogalanin gene. The mouse gene has an exon:intron organisation very similar to that of the rat and human genes. The first exon is noncoding while exons 2–5 carry the coding...

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Bibliographic Details
Published in:Gene 1996-12, Vol.182 (1), p.71-75
Main Authors: Koffer, Barbara, Liu, Marjorie L., Jacoby, Arie S., Shine, John, Iismaa, Tiina P.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA, Complementary - chemistry
Galanin - chemistry
Genomic organisation
Mice
Molecular Sequence Data
Nucleotide sequence
Polymerase Chain Reaction
Protein Precursors - chemistry
Protein Processing, Post-Translational - genetics
Sequence Alignment
Sequence Analysis
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Summary:Using a probe obtained by PCR amplification from mouse genomic DNA, a genomic clone was isolated covering the entire mouse preprogalanin gene. The mouse gene has an exon:intron organisation very similar to that of the rat and human genes. The first exon is noncoding while exons 2–5 carry the coding region. Exon 6 also encodes the stop codon and a polyadenylation signal. The deduced amino-acid sequence of mouse preprogalanin is 94% and 68% identical to the rat and human peptide, respectively. The amino-acid sequence of mouse galanin was confirmed by RT-PCR amplification of mouse brain RNA. The cloning of the mouse galanin gene should allow elucidation of the regulatory characteristics of its promoter and facilitate transgenic approaches to the analysis of galanin gene function in this species.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(96)00477-5