Attempts to initiate cell cultures from Penaeus chinensis tissues

Leibovitz's L-15 medium, Grace's insect medium and a newly designed medium for penaeid shrimp (MPS) were compared for their ability to grow cells in culture from Penaeus chinensis. The medium MPS gave the best results with lymphoid and heart cells forming monolayers in 3 days at 22 °C. Gra...

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Bibliographic Details
Published in:Aquaculture 1996-12, Vol.147 (3), p.151-157
Main Authors: Tong, Shang-liang, Miao, Hong-Zhi
Format: Article
Language:English
Subjects:
ANIMAL VIRUSES
Aquaculture
CELL CULTURE
Cell cultures
Cells
CULTIVO DE CELULAS
CULTURE DE CELLULE
CULTURE MEDIA
Media
Media supplements
MEDIO DE CULTIVO
MILIEU DE CULTURE
PENAEUS
Penaeus chinensis
Shellfish
Tissue
VIRUS DE LOS ANIMALES
VIRUS DES ANIMAUX
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Summary:Leibovitz's L-15 medium, Grace's insect medium and a newly designed medium for penaeid shrimp (MPS) were compared for their ability to grow cells in culture from Penaeus chinensis. The medium MPS gave the best results with lymphoid and heart cells forming monolayers in 3 days at 22 °C. Grace's insect medium was the least effective. There were many cells which migrated from the ovary tissue, and fewer cells from the cerebral ganglion and eye tissues. Fetal bovine serum was essential for cell growth, shrimp muscle extract was less effective and the hemolymph of the mantis shrimp Squilla oratoria inhibited the cells.
ISSN:0044-8486
1873-5622
DOI:10.1016/S0044-8486(96)01386-5