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The level of a mRNA with sequence similarity to the old yellow enzyme-NADPH dehydrogenase increases in Chenopodium rubrum cells in response to cytokinin
Stationary-phase cells of Chenopodium rubrum were treated with t-zeatin riboside for 2 h, and the pattern of gene expression in both treated and non-treated cells was examined by differential display of mRNA. Based on this screen, we isolated a cDNA clone whose corresponding mRNA level increased upo...
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Published in: | Journal of plant physiology 1996, Vol.149 (1), p.233-236 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Stationary-phase cells of
Chenopodium rubrum were treated with
t-zeatin riboside for 2 h, and the pattern of gene expression in both treated and non-treated cells was examined by differential display of mRNA. Based on this screen, we isolated a cDNA clone whose corresponding mRNA level increased upon cytokinin treatment. Northern blot analysis confirmed that expression of this mRNA was induced by
t-zeatin riboside. The amino acid sequence deduced from the partial cDNA showed a high level of similarity to the corresponding amino acid sequence of the old yellow enzyme-NADPH dehydrogenases of fungi. |
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ISSN: | 0176-1617 1618-1328 |
DOI: | 10.1016/S0176-1617(96)80202-1 |