Physical factors negatively affecting evaluation of long-term biodegradation experiments of polychlorinated biphenyls

• Published in: Chemosphere (Oxford) 1996-12, Vol.33 (12), p.2411-2421
• Main Authors: Zachǎr, P., Novotny̌, Č., Vozňáková, Z., Matucha, M., Tesařová, E., Sýkora, D., Kubátová, A., Popl, M., Šašek, V.
• Format: Article
• Language: English
• Subjects: Benzyl Alcohols - chemistry; Biodegradation of pollutants; Biodegradation, Environmental; Biological and medical sciences; Biotechnology; Carbon Radioisotopes; Chromatography, Gas; Culture Media; Environment and pollution; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Isotope Labeling; Mutagens - metabolism; Polychlorinated Biphenyls - metabolism; Polysorbates - chemistry; Structure-Activity Relationship
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/S0045-6535(96)00338-4

Behaviour of PCB added in the form of a technical mixture (Delor 106) and [ 14C]PCB 77 into different aqueous model cultivation media in long-term experiments was studied. The work was focused on monitoring PCB recovery from liquid media in the absence of micro-organisms. GC-ECD and radioanalysis were utilised for determination of PCB in the examined systems. Strong relations between duration of experiments, composition of media, degree of chlorination of biphenyls, and recovery of PCB were found. The lowest yields of PCB were observed for the extracts from model solutions based on N-limited cultivation media without veratryl alcohol and Tween 80. The addition of these components, mainly of Tween 80, caused a significant increase of PCB recoveries.