Production of structured lipid containing docosahexaenoic and caprylic acids using immobilized Rhizopus delemar lipase

Triglycerides with medium-chain fatty acids at the 1- and 3-positions and functional fatty acids at the 2-position are referred to as structured lipids. An attempt was made to produce a structured lipid containing docosahexaenoic and caprylic acids using 1,3-positional specific Rhizopus delemar lipa...

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Bibliographic Details
Published in:Journal of fermentation and bioengineering 1996-01, Vol.81 (4), p.299-303
Main Authors: Shimada, Yuji, Sugihara, Akio, Maruyama, Kazuaki, Nagao, Toshihiro, Nakayama, Suguru, Nakano, Hirofumi, Tominaga, Yoshio
Format: Article
Language:English
Subjects:
ACIDE CAPRYLIQUE
ACIDE GRAS POLYINSATURE
ACIDO CAPRILICO
acidolysis
ACIDOS GRASOS POLIINSATURADOS
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
docosahexaenoic acid
ENZIMAS INMOVILIZADAS
ENZYME IMMOBILISEE
Fundamental and applied biological sciences. Psychology
immobilized lipase
LIPIDE
LIPIDOS
Methods. Procedures. Technologies
RHIZOPUS
Rhizopus delemar
structured lipid
TRIACILGLICEROL LIPASA
TRIACYLGLYCEROL LIPASE
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Summary:Triglycerides with medium-chain fatty acids at the 1- and 3-positions and functional fatty acids at the 2-position are referred to as structured lipids. An attempt was made to produce a structured lipid containing docosahexaenoic and caprylic acids using 1,3-positional specific Rhizopus delemar lipase, which was immobilized on a ceramic carrier by coprecipitation with acetone. When a reaction mixture containing 5 g tuna oil and 10 g caprylic acid, 0.6 g immobilized lipase, and 0.3 g water was incubated at 30°C for 2 d with shaking, acidolysis proceeded effectively but hydrolysis occurred simultaneously. This hydrolysis could be repressed by omitting water from the subsequent reaction mixture. The immobilized lipase could be reused 14 times without any significant loss of activity, and approximately 65% of the fatty acids at the 1- and 3-positions in the tuna oil were exchanged for caprylic acid. All the triglyceride products contained one or two caprylic acid molecule(s). Docosahexaenoic acid bound at the 1(3)-position in tuna oil was not exchanged because of the low activity of the R. delemar lipase on it.
ISSN:0922-338X
DOI:10.1016/0922-338X(96)80580-0