Mutants of Pseudomonas fluorescens NCIMB 11671 defective in the catabolism of alpha-pinene

Pseudomonas fluorescens NCIMB 11671 metabolises alpha-pinene via alpha-pinene oxide and 2-methyl-5-isopropylhexa-2,5-dienal. Mutants unable to grow on alpha-pinene and/or alpha-pinene oxide have been isolated by N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis, including an unexpected phenotype...

Full description

Saved in:
Bibliographic Details
Published in:Applied microbiology and biotechnology 1996-07, Vol.45 (6), p.822-830
Main Authors: Colocousi, A, Saqib, K.M, Leak, D.J
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Biological Sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Mission oriented research
Physiology and metabolism
Pseudomonas fluorescens
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pseudomonas fluorescens NCIMB 11671 metabolises alpha-pinene via alpha-pinene oxide and 2-methyl-5-isopropylhexa-2,5-dienal. Mutants unable to grow on alpha-pinene and/or alpha-pinene oxide have been isolated by N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis, including an unexpected phenotype able to grow on alpha-pinene but not on alpha-pinene oxide. The mutants have been classified on the basis of their alpha-pinene monooxygenase, alpha-pinene oxide lyase and aldehyde dehydrogenase activities. Biotransformation of alpha-pinene by the wild-type and mutant strains has revealed evidence for alternative routes for pinene metabolism to that already proposed.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530050769