Engineering new functions and altering existing functions

Structural and mechanistic information, sequence comparisons, and site-directed mutagenesis data continue to provide a basis for the rational design of new protein functions and the alteration of existing functions. Random mutagenesis and ‘directed evolution’ approaches, however, are making signific...

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Published in:Current opinion in structural biology 1996-08, Vol.6 (4), p.513-518
Main Authors: Shao, Zhixin, Arnold, Frances H
Format: Article
Language:English
Subjects:
Binding Sites
Directed Molecular Evolution
Green Fluorescent Proteins
Luminescent Proteins - chemistry
Protein Engineering
Proteins - chemistry
Proteins - metabolism
Structure-Activity Relationship
Substrate Specificity
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description Structural and mechanistic information, sequence comparisons, and site-directed mutagenesis data continue to provide a basis for the rational design of new protein functions and the alteration of existing functions. Random mutagenesis and ‘directed evolution’ approaches, however, are making significant headway in solving protein engineering problems, proving highly practical for tuning properties such as enzyme substrate specificity.
doi_str_mv 10.1016/S0959-440X(96)80117-5
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source ScienceDirect Journals
subjects Binding Sites
Directed Molecular Evolution
Green Fluorescent Proteins
Luminescent Proteins - chemistry
Protein Engineering
Proteins - chemistry
Proteins - metabolism
Structure-Activity Relationship
Substrate Specificity
title Engineering new functions and altering existing functions
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