Medium optimization for recombinant protein production by Bacillus subtilis

Optimal concentrations of glucose, yeast extract and nutrient broth for the production of beta -lactamase by B. subtilis were predicted using a statistical experimental design and then tested. More yeast extract (13 vs. 1 g/L), less glucose (7.4 vs. 10 g/L), and less nutrient broth (12.6 vs. 15 g/L)...

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Bibliographic Details
Published in:Biotechnology letters 1996-06, Vol.18 (6), p.737-740
Main Authors: PARK, K, REARDON, K. F
Format: Article
Language:English
Subjects:
Bacillus subtilis
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
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Summary:Optimal concentrations of glucose, yeast extract and nutrient broth for the production of beta -lactamase by B. subtilis were predicted using a statistical experimental design and then tested. More yeast extract (13 vs. 1 g/L), less glucose (7.4 vs. 10 g/L), and less nutrient broth (12.6 vs. 15 g/L) were required to achieve high beta -lactamase activities (5700 U/L) instead of high cell growth rates (1.2 h super(-1)).
ISSN:0141-5492
1573-6776
DOI:10.1007/bf00130775