DNA typing of hops (Humulus lupulus) through application of RAPD and microsatellite marker sequences converted to sequence tagged sites (STS)

Both random amplified polymorphic DNA and microsatellite repeat sequences were investigated as DNA markers for distinguishing hop cultivars. Microsatellite sequences converted to STS markers proved to be most successful. The relative abundance of microsatellite repeat sequences in the hop genome var...

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Bibliographic Details
Published in:Euphytica 1996, Vol.91 (3), p.277-284
Main Authors: Brady, J.L. (Division of Horticulture SCIRO, Adelaide, S.A. (Australia)), Scott, N.S, Thomas, M.R
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biotechnology
Cultivars: description, identification, tests, catalogs
Fundamental and applied biological sciences. Psychology
GENETIC MARKERS
Genetics and breeding of economic plants
HUMULUS LUPULUS
MARCADORES GENETICOS
MARQUEUR GENETIQUE
NUCLEOTIDE SEQUENCE
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Varietal selection. Specialized plant breeding, plant breeding aims
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Summary:Both random amplified polymorphic DNA and microsatellite repeat sequences were investigated as DNA markers for distinguishing hop cultivars. Microsatellite sequences converted to STS markers proved to be most successful. The relative abundance of microsatellite repeat sequences in the hop genome varied depending on the sequence class. Of the repeat types investigated the dinucleotide repeats (GA) sub(n) and (GT) sub(n) are the most highly represented in the hop genome. Microsatellite repeat sequences in hops have been shown to be highly polymorphic and are very informative as STS molecular markers. A DNA typing system using sequence-tagged microsatellite site markers has been developed which will not only be useful for hop cultivar identification but also marker assisted breeding and quality control purposes.
ISSN:0014-2336
1573-5060
DOI:10.1007/BF00033088