Persistent chemopreventive effect of S-adenosyl-L-methionine on the development of liver putative preneoplastic lesions induced by thiobenzamide in diethylnitrosamine-initiated rats

• Published in: Carcinogenesis (New York) 1996-07, Vol.17 (7), p.1533-1538
• Main Authors: SIMILE, M. M, SAVIOZZI, M, DE MIGLIO, M. R, MURONI, M. R, NUFRIS, A, PASCALE, R. M, MALVADI, G, FEO, F
• Format: Article
• Language: English
• Subjects: Animal tumors. Experimental tumors; Animals; Anticarcinogenic Agents - pharmacology; Biological and medical sciences; Carcinogens; Diethylnitrosamine; DNA - biosynthesis; Experimental digestive system and abdominal tumors; gamma-Glutamyltransferase - metabolism; Gene Expression - drug effects; Genes, myc; Genes, ras; Liver - drug effects; Liver - metabolism; Liver - pathology; Liver Neoplasms - chemically induced; Liver Neoplasms - pathology; Liver Neoplasms - prevention & control; Male; Medical sciences; Ornithine Decarboxylase - metabolism; Precancerous Conditions - chemically induced; Precancerous Conditions - pathology; Precancerous Conditions - prevention & control; Rats; Rats, Inbred F344; RNA, Messenger - analysis; S-Adenosylmethionine - pharmacology; Thioamides; Time Factors; Tumors
• ISSN: 0143-3334; 1460-2180
• DOI: 10.1093/carcin/17.7.1533

S-Adenosyl-L-methionine (SAM) is a strong chemopreventive agent of rat liver carcinogenesis. Examination was made to determine whether inhibition by SAM of the development of preneoplastic liver lesions persists to SAM withdrawal in diethylnitrosamine-initiated F344 rats promoted with thiobenzamide (TB). The rats were subjected, 2 weeks after initiation, to 5 weeks feeding with a 0.1% TB diet followed by a TB-free diet for 6 weeks and then a second TB treatment for 3 weeks. SAM (384 micromol/kg/day) was injected i.m. during the first TB cycle (treatment A) or for 6 weeks after the first TB cycle (treatment B). Many gamma-glutamyltranspeptidase (GGT)-positive lesions developed in initiated rats after the first TB cycle. They decreased in number after TB withdrawal, while partial recovery of lesion number and a great increase in volume occurred after the second TB cycle. Liver ornithine decarboxylase (ODC) activity and c-myc and c-Ha-ras mRNAs increased during the TB cycles and returned to normal liver values after TB withdrawal. Number and size of GGT-positive lesions, DNA synthesis of GGT-positive cells, liver ODC activity and c-myc and c-Ha-ras mRNA levels decreased as a consequence of SAM treatment A. The recovery of these parameters, induced by a second TB cycle in rats not treated with SAM, was prevented by SAM treatment B. These results suggest that SAM causes a persistent decrease in growth capacity of preneoplastic liver lesions in rats subjected to a diethylnitrosamine/TB protocol.