Comparison of three different tests for detection of cucumber mosaic cucumovirus in banana (Musa paradisiaca)

Direct ELISAs like double antibody sandwich (DAS)–ELISA, simplified rapid direct antigen coating (SRDAC)–ELISA and indirect form of DAC– ELISA for detection of cucumber mosaic cucumovirus (CMV) causing infectious chlorosis of banana in leaf extracts and pseudostem sap exudates and purified virus dil...

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Bibliographic Details
Published in:Current science (Bangalore) 1996-11, Vol.71 (10), p.764-767
Main Authors: Kiranmai, G., Sreenivasulu, P., Nayudu, M. V.
Format: Article
Language:English
Subjects:
Bioassay
cucumber mosaic virus
Double stranded RNA
Leaves
Lesions
Mosaic
Musa paradisiaca
Plant antigens
Plant diseases
Pseudostems
RESEARCH COMMUNICATIONS
Sap
Viruses
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Summary:Direct ELISAs like double antibody sandwich (DAS)–ELISA, simplified rapid direct antigen coating (SRDAC)–ELISA and indirect form of DAC– ELISA for detection of cucumber mosaic cucumovirus (CMV) causing infectious chlorosis of banana in leaf extracts and pseudostem sap exudates and purified virus diluted with antigen buffer or healthy banana leaf sap were compared. The sensitivity levels of three ELISA tests were similar and the virus was detected up to 10-7 dilution with leaf extract, 1 μl/well with sap exudate, 20 ng/well with purified CMV and 100 ng/well with purified virus diluted with healthy banana leaf extract. Of three forms of ELISA compared, DAC-ELISA was further evaluated with detection of infectious virus by local lesion bioassay on cowpea (Vigna unguiculata) and by double stranded RNA (dsRNA) analysis. The banana leaf and pseudostem sap exudate samples that were positive in DAC-ELISA were also positive by other two tests. Collection of pseudostem sap exudates by pin pricking from test plants and detection of virus by DAC-ELISA in them appears ideal for large-scale testing of banana plants.
ISSN:0011-3891