Non-covalent enzyme-polyelectrolyte complexes as self-buffered catalysts for use in low-water organic media

When free chymotrypsin is used to catalyse hydrolysis of N-acetyl tyrosine ethyl ester in 90% acetonitrile, the reaction rate soon falls because of the accumulation of the acidic product. If the enzyme is used in the form of a suspended complex with polyacrylic acid, the polyelectrolyte acts as an a...

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Bibliographic Details
Published in:Biotechnology techniques 1996-08, Vol.10 (8), p.621-624
Main Authors: Vakurov, Alexander V., Gladilin, Alexander K., Partridge, Johann, Mozhaev, Vadim V., Levashov, Andrey V., Halling, Peter J.
Format: Article
Language:English
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Summary:When free chymotrypsin is used to catalyse hydrolysis of N-acetyl tyrosine ethyl ester in 90% acetonitrile, the reaction rate soon falls because of the accumulation of the acidic product. If the enzyme is used in the form of a suspended complex with polyacrylic acid, the polyelectrolyte acts as an acid-base buffer to permit extended reaction.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00157373