Loading…
Quantitative detection of messenger RNA by solution hybridization and enzyme immunoassay
A novel nucleic acid detection technique is described for the quantitative measurement of eukaryotic mRNA in biological samples. The procedure involves two steps: a hybridization reaction in solution with a biotinylated cDNA probe, and a conventional enzyme immunoassay that uses a monoclonal antibod...
Saved in:
Published in: | The Journal of biological chemistry 1990-07, Vol.265 (20), p.11601-11604 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | A novel nucleic acid detection technique is described for the quantitative measurement of eukaryotic mRNA in biological samples.
The procedure involves two steps: a hybridization reaction in solution with a biotinylated cDNA probe, and a conventional
enzyme immunoassay that uses a monoclonal antibody for DNA.RNA hybrids to detect the specific mRNA.cDNA complexes. The method
has comparable sensitivity to 32P-based methods and yields results that are quantitative and highly reproducible. Furthermore,
the test can be performed using unfractionated cytoplasm without the need for extraction with organic solvents. This technique
provides a rapid and quantitative method for studying changes in cellular mRNA levels, and it is suitable for testing large
numbers of samples. |
---|---|
ISSN: | 0021-9258 1083-351X |
DOI: | 10.1016/S0021-9258(19)38440-6 |