Foetal ventral mesencephalic cell suspension grafts to the 6-hydroxydopamine-lesioned rat reduce the rate of dopamine uptake in the contralateral striatum

The present study employed fast cyclic voltammetry, at carbon-fibre microelectrodes, to monitor and compare the rate of dopamine uptake in the rat striatum contralateral to (a) the 6-hydroxydopamine (6-OHDA)-lesioned/grafted striatum and (b) the 6-OHDA-lesioned/sham grafted striatum. Cell suspension...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience letters 1996-06, Vol.210 (3), p.185-188
Main Authors: Earl, Christopher D., Marburger, Andrea, Schönfuβ, Dirk, Sautter, Jürgen, Strecker, Sabine, Yang, Zhengming, Kupsch, Andreas, Oertel, Wolfgang H., Morgenstern, Rudolf
Format: Article
Language:English
Subjects:
Amphetamine - pharmacology
Animals
Biological and medical sciences
Cell Transplantation - physiology
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Dopamine
Dopamine - metabolism
Dopamine Uptake Inhibitors - pharmacology
Electric Stimulation
Fast cyclic voltammetry
Female
Fetal Tissue Transplantation - physiology
Functional Laterality - physiology
High affinity uptake
In vivo
Kinetics
Medical sciences
Mesencephalon - transplantation
Microelectrodes
Neostriatum - metabolism
Neostriatum - physiology
Neural Grafting
Neurology
Non-grafted side
Oxidopamine - toxicity
Parkinson's disease
Piperazines - pharmacology
Rats
Rats, Sprague-Dawley
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The present study employed fast cyclic voltammetry, at carbon-fibre microelectrodes, to monitor and compare the rate of dopamine uptake in the rat striatum contralateral to (a) the 6-hydroxydopamine (6-OHDA)-lesioned/grafted striatum and (b) the 6-OHDA-lesioned/sham grafted striatum. Cell suspensions of foetal rat ventral mesencephalic tissue were grafted into the dopamine-depleted striatum of unilaterally 6-OHDA-lesioned rats. Six weeks after grafting, animals with functional, mature grafts were monitored for dopamine elimination in the contralateral striatum following electrical stimulation of the median forebrain bundle, before and after treatment with the dopamine uptake inhibitor GBR 12909. Compared to animals with sham grafts, amphetamine-amplified rotational behaviour was significantly reduced in animals with grafts of foetal ventral mesencephalic tissue. Fast cyclic voltammeric measurements followed by evaluation with the aid of a kinetic model revealed that in grafted animals, the rate of dopamine uptake via the high affinity uptake mechanism, following treatment with GBR 12909, was significantly reduced when compared to sham grafted animals.
ISSN:0304-3940
1872-7972
DOI:10.1016/0304-3940(96)12695-1