Secretion of thermostable β-glucosidase by an intergeneric bacterial hybrid between Cellulomonas and Bacillus subtilis

The intergeneric protoplast fusion hybrid (Bs/C 005) between Cellulomonas sp. and Bacillus subtilis produced extracellular aryl beta -glucosidase that is otherwise intracellular in parental Cellulomonas sp. This extracellular aryl beta -glucosidase was active at relatively higher temperature (60 deg...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1990-12, Vol.26 (3), p.207-215
Main Authors: GOKHALE, D. V, DEOBAGKAR, D. N
Format: Article
Language:English
Subjects:
beta -glucosidase
Biological and medical sciences
Biotechnology
Cell fusion (prokaryotes)
Cellulomonas
extracellular enzymes
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
thermal stability
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Summary:The intergeneric protoplast fusion hybrid (Bs/C 005) between Cellulomonas sp. and Bacillus subtilis produced extracellular aryl beta -glucosidase that is otherwise intracellular in parental Cellulomonas sp. This extracellular aryl beta -glucosidase was active at relatively higher temperature (60 degree C) and lower pH (pH 5.0) conditions than that of Cellulomonas enzyme. It also exhibited increased thermostability and stability over wide range of pH. Cellobiase activity, distinctly different from aryl beta -glucosidase detected in both Cellulomonas sp. Bs/C 005, was only intracellular. Cellobiase from Bs/C 005, however, was more thermostable than that of Cellulomonas sp.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02921502