Ammonium photoproduction by free and immobilized cells of Chlamydomonas reinhardtii

Free-living or immobilized Chlamydomonas reinhardtii cells photoproduce ammonium from nitrite in a medium containing 1 mM of L-methionine-D,L-sulphoximine (MSX). Ammonium is accumulated in the medium to 8 mM final concentration, which inhibits nitrite uptake by the MSX-treated cells and consequently...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1989-07, Vol.31 (1), p.55-58
Main Authors: Santos-Rosa, F, Galvan, F
Format: Article
Language:English
Subjects:
ammonia
ammonium
aquatic plants
Biological and medical sciences
biosynthesis
Biotechnology
Chlamydomonas reinhardtii
Chlorophyta
Fundamental and applied biological sciences. Psychology
immobilization
Immobilization of organelles and whole cells
Immobilization techniques
immobilized cells
L-methionine-DL-sulfoximine
light
light relations
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
photoinduction
photosynthesis
plant physiology
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Summary:Free-living or immobilized Chlamydomonas reinhardtii cells photoproduce ammonium from nitrite in a medium containing 1 mM of L-methionine-D,L-sulphoximine (MSX). Ammonium is accumulated in the medium to 8 mM final concentration, which inhibits nitrite uptake by the MSX-treated cells and consequently the excretion of ammonium is blocked. However, if ammonium was removed from the medium and nitrite and MSX periodically restored, the photoproduction process could be maintained over 96 h, with a final ammonium concentration of about 18 mM for free-living cells and 28 mM for immobilized ones. The MSX-treated cells showed a photoproduction productivity of 1300 mu mol NH sub(4) super(+) multiplied by mg chlorophyll/(Chl), with an average production rate of 14 mu mol NH sub(4) super(+) multiplied by mg/Chl per hour, for calcium alginate-entrapped cells.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00252526