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Development of a colony-blot ELISA assay using monoclonal antibodies to identify Vibrio P1 responsible for “brown ring disease”: in the clam Tapes philippinarum

Since 1987, brown ring disease has caused mass mortalities on various cultured clam-beds. The causal agent is a Vibrio sp. called Vibrio P1. The problem is to perform a suitable, reliable, rapid and sensitive technique to detect this bacterium in the environment. Therefore, monoclonal antibodies (mA...

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Bibliographic Details
Published in:Aquaculture 1996-11, Vol.146 (3), p.171-178
Main Authors: Noel, T., Nicolas, J.-L., Boulo, V., Mialhe, E., Roch, Ph
Format: Article
Language:English
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Summary:Since 1987, brown ring disease has caused mass mortalities on various cultured clam-beds. The causal agent is a Vibrio sp. called Vibrio P1. The problem is to perform a suitable, reliable, rapid and sensitive technique to detect this bacterium in the environment. Therefore, monoclonal antibodies (mAb) were prepared according to hybridoma technology. One mAb was selected by enzyme-linked immunosorbent assay (ELISA) because of its capacity to react specifically with V. P1. Nitro-cellulose membrane immunoassay (colony-blot ELISA) was developed to detect directly V P1 in samples. Results of identification of 223 bacteria by the biochemical method and by the colony-blot ELISA method were compared. The specificity and the sensitivity were 100% in both cases. Our results indicate that the colony-blot ELISA is sensitive, easy to perform and is especially suited for routine diagnostic work.
ISSN:0044-8486
1873-5622
DOI:10.1016/S0044-8486(96)01385-3