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Development of a colony-blot ELISA assay using monoclonal antibodies to identify Vibrio P1 responsible for “brown ring disease”: in the clam Tapes philippinarum
Since 1987, brown ring disease has caused mass mortalities on various cultured clam-beds. The causal agent is a Vibrio sp. called Vibrio P1. The problem is to perform a suitable, reliable, rapid and sensitive technique to detect this bacterium in the environment. Therefore, monoclonal antibodies (mA...
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Published in: | Aquaculture 1996-11, Vol.146 (3), p.171-178 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Since 1987, brown ring disease has caused mass mortalities on various cultured clam-beds. The causal agent is a
Vibrio sp. called
Vibrio P1. The problem is to perform a suitable, reliable, rapid and sensitive technique to detect this bacterium in the environment. Therefore, monoclonal antibodies (mAb) were prepared according to hybridoma technology. One mAb was selected by enzyme-linked immunosorbent assay (ELISA) because of its capacity to react specifically with
V. P1. Nitro-cellulose membrane immunoassay (colony-blot ELISA) was developed to detect directly
V P1 in samples. Results of identification of 223 bacteria by the biochemical method and by the colony-blot ELISA method were compared. The specificity and the sensitivity were 100% in both cases. Our results indicate that the colony-blot ELISA is sensitive, easy to perform and is especially suited for routine diagnostic work. |
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ISSN: | 0044-8486 1873-5622 |
DOI: | 10.1016/S0044-8486(96)01385-3 |