Development of a colony-blot ELISA assay using monoclonal antibodies to identify Vibrio P1 responsible for “brown ring disease”: in the clam Tapes philippinarum

Since 1987, brown ring disease has caused mass mortalities on various cultured clam-beds. The causal agent is a Vibrio sp. called Vibrio P1. The problem is to perform a suitable, reliable, rapid and sensitive technique to detect this bacterium in the environment. Therefore, monoclonal antibodies (mA...

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Published in:Aquaculture 1996-11, Vol.146 (3), p.171-178
Main Authors: Noel, T., Nicolas, J.-L., Boulo, V., Mialhe, E., Roch, Ph
Format: Article
Language:English
Subjects:
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
Aquaculture
Bacteria
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
Disease
ELISA
Fish
Mollusks
MONOCLONAL ANTIBODIES
TAPES
TAPES (GENERO)
TAPES (GENUS)
Tapes philippinarum
Tapes philippinarum Brown ring disease
TEST ELISA
VIBRIO
Vibrio P1
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container_title Aquaculture
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creator Noel, T.
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description Since 1987, brown ring disease has caused mass mortalities on various cultured clam-beds. The causal agent is a Vibrio sp. called Vibrio P1. The problem is to perform a suitable, reliable, rapid and sensitive technique to detect this bacterium in the environment. Therefore, monoclonal antibodies (mAb) were prepared according to hybridoma technology. One mAb was selected by enzyme-linked immunosorbent assay (ELISA) because of its capacity to react specifically with V. P1. Nitro-cellulose membrane immunoassay (colony-blot ELISA) was developed to detect directly V P1 in samples. Results of identification of 223 bacteria by the biochemical method and by the colony-blot ELISA method were compared. The specificity and the sensitivity were 100% in both cases. Our results indicate that the colony-blot ELISA is sensitive, easy to perform and is especially suited for routine diagnostic work.
doi_str_mv 10.1016/S0044-8486(96)01385-3
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subjects ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
Aquaculture
Bacteria
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
Disease
ELISA
Fish
Mollusks
MONOCLONAL ANTIBODIES
TAPES
TAPES (GENERO)
TAPES (GENUS)
Tapes philippinarum
Tapes philippinarum Brown ring disease
TEST ELISA
VIBRIO
Vibrio P1
title Development of a colony-blot ELISA assay using monoclonal antibodies to identify Vibrio P1 responsible for “brown ring disease”: in the clam Tapes philippinarum
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