Effects of joining peptide (1-18) and histamine on dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulphate (DHEAS) production of human adrenocortical cells in vitro

• Published in: The Journal of steroid biochemistry and molecular biology 1996-05, Vol.58 (2), p.207-210
• Main Authors: Orsó, Evelyn, Szalay, K.Sz, Szabó, D., Stark, E., Fehér, T., Perner, F., Hidvégi, M.
• Format: Article
• Language: English
• Subjects: Adrenal Cortex - cytology; Adrenal Cortex - drug effects; Adrenal Cortex - metabolism; Adrenals. Interrenals; Adrenocortical hormones. Regulation; Adrenocorticotropic Hormone - pharmacology; Amino Acid Sequence; Biological and medical sciences; Dehydroepiandrosterone - metabolism; Dehydroepiandrosterone Sulfate - metabolism; Drug Synergism; Fundamental and applied biological sciences. Psychology; Histamine - pharmacology; Humans; In Vitro Techniques; Molecular Sequence Data; Peptide Fragments - pharmacology; Pro-Opiomelanocortin - pharmacology; Vertebrates: endocrinology
• ISSN: 0960-0760; 1879-1220
• DOI: 10.1016/0960-0760(96)00027-1

Joining peptide 1-18 (JP 1-18), added alone in concentrations of 10−13–10−7 M to collagenase-dispersed human adrenocortical cells, did not affect the basal production of corticosterone, cortisol, aldosterone, dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulphate (DHEAS). JP 1-18 potentiated the ACTH-stimulated production of steroids. When administered in combination with histamine (10−8–10−3 M), JP 1-18 (10−8 or 10−10 M), enhanced the synthesis of DHEA and DHEAS. JP 1-18, together with histamine, may play a role in the regulation of DHEA and DHEAS production.