Bacillus stearothermophilus spores on strips. Calibration of thermoresistance kinetics to prior biological validation

Filter-paper strips saturated with Bacillus stearothermophilus spores were prepared and then stored for up to 1470 days. The strips were used to measure spore activation time and death kinetics in two systems: a serum bottle/oil bath combination, and glassine packages in a gravity autoclave. The dat...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1996-11, Vol.46 (4), p.340-346
Main Authors: VESSONI PENNA, T. C, MACHOSHVILI, I. A, AQUARONE, E
Format: Article
Language:English
Subjects:
Bacillus stearothermophilus
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
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Summary:Filter-paper strips saturated with Bacillus stearothermophilus spores were prepared and then stored for up to 1470 days. The strips were used to measure spore activation time and death kinetics in two systems: a serum bottle/oil bath combination, and glassine packages in a gravity autoclave. The data collected were analyzed primarily by analysis of variance and the interaction of spore storage time, processing temperature, inertial time and exposure time was determined.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00166227