Molecular analysis of a spontaneous insertion mutation near the alcohol dehydrogenase gene of Drosophila melanogaster

The structural integrity of the Adh gene in several isogenic lines of Drosophila melanogaster was tested by Southern blot analysis using a 4.75 kilobase (kb) genomic clone of Alcohol dehydrogenase (Adh) as a probe. One line, RI22, III, showed evidence of a spontaneous insertion mutation 5′ to the ad...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 1996-06, Vol.26 (6), p.593-598
Main Authors: White, Lisa D., Jacobson, James W.
Format: Article
Language:English
Subjects:
Alcohol dehydrogenase
Alcohol Dehydrogenase - genetics
alleles
Animals
Base Sequence
DNA - analysis
Drosophila melanogaster
Drosophila melanogaster - enzymology
enhancer sequences
Female
genbank/u35852
gene frequency
genetic techniques and protocols
Jockey
jockey retrotransposon
Male
Molecular Sequence Data
Mutagenesis, Insertional
mutants
mutation
Restriction Mapping
retrotransposons
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
structural genes
Transposable elements
transposons
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Summary:The structural integrity of the Adh gene in several isogenic lines of Drosophila melanogaster was tested by Southern blot analysis using a 4.75 kilobase (kb) genomic clone of Alcohol dehydrogenase (Adh) as a probe. One line, RI22, III, showed evidence of a spontaneous insertion mutation 5′ to the adult enhancer in an area previously indicated as a putative larval enhancer region. The inserted allele was present at an approximate frequency of 50% in relation to the uninserted wildtype allele. Isogenic lines were constructed of both homozygous mutant and wildtype flies, allowing the comparison of a spontaneous insertion mutant allele and it's direct wildtype ancestral allele. The inserted sequence is a 296 basepair (bp) truncated jockey retroposable element. The sequence and distribution of the element as well as it's proximity to the Adh gene are discussed.
ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(96)00018-5