Multiple forms of G sub(o alpha ) mRNA: Analysis of the 3'-untranslated regions

• Published in: Biochemistry (Easton) 1990-01, Vol.29 (21), p.5069-5076
• Main Authors: Price, SR, Murtagh, JJ Jr, Tsuchiya, M, Serventi, I M, Van Meurs, KP, Angus, C W, Moss, J, Vaughan, M
• Format: Article
• Language: English
• ISSN: 0006-2960

G sub(o), a guanine nucleotide binding protein found predominantly in neural tissues, interacts in vitro with rhodopsin, muscarinic, and other receptors and has been implicated in the regulation of ion channels. Despite the virtual identity of reported cDNA sequences for the alpha subunit of G sub(o) (G sub(o alpha )), multiple molecular weight forms of mRNA have been identified in tissues from all species examined. To investigate the molecular basis for the size heterogeneity of G sub(o alpha ) mRNAs, four cDNA clones were isolated from the same retinal lambda gt10 cDNA library that was used earlier to isolate lambda GO9, a clone encompassing the complete coding region of G sub(o alpha ). These clones were identified as G sub(o alpha ) clones based on nucleotide sequence identity with lambda GO9 in the coding region; they diverge, however, from lambda GO9 in the 3'-untranslated region 28 nucleotides past the stop codon.