TNF-α, IL-6, and Leptin Increase the Expression of miR-378, an Adipogenesis-Related microRNA in Human Adipocytes

Obesity has become a global public health problem associated with complications including type 2 diabetes, cardiovascular disease, and several cancers. Adipocyte differentiation (adipogenesis) plays an important role in obesity and energy homeostasis. Adipose tissue secretes multiple cytokines and a...

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Bibliographic Details
Published in:Cell biochemistry and biophysics 2014-11, Vol.70 (2), p.771-776
Main Authors: Xu, Lu-lian, Shi, Chun-mei, Xu, Guang-feng, Chen, Ling, Zhu, Ling-ling, Zhu, Lu, Guo, Xi-rong, Xu, Mei-yu, Ji, Chen-bo
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - drug effects
Adipocytes - metabolism
Adipogenesis - drug effects
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biotechnology
Cell Biology
Humans
Interleukin-6 - pharmacology
Leptin - pharmacology
Life Sciences
MicroRNAs - genetics
Original Paper
Pharmacology/Toxicology
Tumor Necrosis Factor-alpha - pharmacology
Up-Regulation - drug effects
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Summary:Obesity has become a global public health problem associated with complications including type 2 diabetes, cardiovascular disease, and several cancers. Adipocyte differentiation (adipogenesis) plays an important role in obesity and energy homeostasis. Adipose tissue secretes multiple cytokines and adipokines which can cause the complications of obesity, especially insulin resistance. TNF-α, IL-6, leptin, and resistin have been identified as the main regulators of obesity and insulin activity. miR-378 is highly induced during adipogenesis and has been reported to be positively regulated in adipogenesis. In the current study, matured human adipocytes were treated with TNF-α, IL-6, leptin, or resistin on the 15th day after the induction of human pre-adipocyte differentiation. We demonstrated that TNF-α, IL-6, and leptin upregulated miR-378 expression indicating that miR-378 probably is a novel mediator in the development of insulin resistance related to obesity.
ISSN:1085-9195
1559-0283
DOI:10.1007/s12013-014-9980-x