The effect of colchicine and low-dose methotrexate on intestinal ischemia/reperfusion injury in an experimental model

Abstract Aim Intestinal ischemia/reperfusion (I/R) injury is a serious clinical condition. Colchicine and low-dose methotrexate have anti-inflammatory features. An experimental model was conducted to investigate the effect of colchicine and methotrexate on intestinal I/R injury. Methods Twenty-four...

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Bibliographic Details
Published in:Journal of pediatric surgery 2014-10, Vol.49 (10), p.1471-1474
Main Authors: Boybeyi, Özlem, Gunal, Yasemin Dere, Atasoy, Pinar, Kısa, Ucler, Aslan, Mustafa Kemal
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents - administration & dosage
Colchicine
Colchicine - administration & dosage
Disease Models, Animal
Experimental
Ileal Diseases - drug therapy
Intestinal ischemia
Intestines - blood supply
Intestines - pathology
Ischemia/reperfusion injury
Male
Methotrexate
Methotrexate - administration & dosage
Pediatrics
Rats
Rats, Wistar
Reperfusion Injury - drug therapy
Surgery
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Summary:Abstract Aim Intestinal ischemia/reperfusion (I/R) injury is a serious clinical condition. Colchicine and low-dose methotrexate have anti-inflammatory features. An experimental model was conducted to investigate the effect of colchicine and methotrexate on intestinal I/R injury. Methods Twenty-four rats were included. Only laparotomy was done in control group (CG, n = 6). In experimental groups, superior mesenteric artery was occluded. After 1 h ischemia, reperfusion (1 h) was started by de-occlusion. 30 min before reperfusion, saline in sham group (SG, n:6), colchicine (1 mg/kg) in colchicine group (CNG, n:6), and methotrexate (0.1 mg/kg) in methotrexate group (MTXG, n:6) were infused intraperitoneally. Small intestines were harvested for evaluation of intestinal mucosal injury (Chiu score) and oxidative stress markers (nitric oxide: NO, malondialdehyde: MDA, superoxide dismutase: SOD). Results Biochemically, MDA levels were significantly low in CG compared to SG, CNG, and MTXG (p < 0.05). NO levels were significantly low and SOD levels were significantly high in CG compared to MTXG (p < 0.05). Histopathologically, Chiu score was significantly low in CG compared to SG, CNG, and MTXG (p < 0.05), and significantly high in MTXG compared to SG and CNG (p < 0.05). Conclusion The present experimental model caused I/R injury in rat intestines. Contrary to literature, it was found that methotrexate worsens and colchicine does not attenuate intestinal I/R injury.
ISSN:0022-3468
1531-5037
DOI:10.1016/j.jpedsurg.2014.01.057