Influence of Biphasic β-TCP with and without the use of collagen membranes on bone healing of surgically critical size defects. A radiological, histological, and histomorphometric study

• Published in: Clinical oral implants research 2014-11, Vol.25 (11), p.1228-1238
• Main Authors: Calvo-Guirado, Jose L., Ramírez-Fernández, Maria P., Delgado-Ruíz, Rafael A., Maté-Sánchez, Jose E., Velasquez, Pablo, de Aza, Piedad N.
• Format: Article
• Language: English
• Subjects: Absorbable Implants; Animals; Bone Density - physiology; Bone Diseases - diagnostic imaging; Bone Diseases - pathology; Bone Diseases - surgery; Bone Regeneration - physiology; bone substitute; Bone Substitutes - therapeutic use; Calcium Phosphates - therapeutic use; Collagen; collagen membrane; critical size defects; Dentistry; guide bone regeneration; Guided Tissue Regeneration - methods; hydroxyapatite; Membranes, Artificial; Osteogenesis - physiology; Rabbits; Radiographic Image Enhancement - methods; Tibia - diagnostic imaging; Tibia - pathology; Tibia - surgery; Time Factors; tricalcium phosphate
• ISSN: 0905-7161; 1600-0501
• DOI: 10.1111/clr.12258

The aim of this study was to investigate, by means of radiological and histomorphometric analysis, the effect of resorbable collagen membranes on critical size defects (CSD) in rabbit tibiae filled with biphasic calcium phosphate. Three CSD of 6 mm diameter were created in both tibiae of 20 New Zealand rabbits and divided into three groups according to the filling material: Group A (Ossceram), Group B (Ossceram plus Alveoprotect membrane), and Group C (unfilled control group). Five animals from each group were sacrificed after 15, 30, 45, and 60 days. Anteroposterior and lateral radiographs were taken. Samples were processed for observation under light microscopy. At the end of treatment, radiological analysis found that cortical defect closure was greater in Group B than Group A, and radiopacity was clearly lower and more heterogeneous in the Group A cortical defects than in Group B. There was no cortical defect closure in Group C. Histomorphometric evaluation showed significant differences in newly formed bone and cortical closure in Group B compared with Groups A and C, with the presence of higher density newly formed bone in cortical and medullar zones. There was no cortical defect closure or medullar bone formation in Group C. Biphasic calcium phosphate functioned well as a scaffolding material allowing mineralized tissue formation. Furthermore, the addiction of absorbable collagen membranes enhanced bone gain compared with non-membrane-treated sites.