Macrophage inhibitory factor-A3 derived from Mycobacterium avium serovar 2 inhibits candidacidal activity of murine peritoneal macrophages

• Published in: Veterinary microbiology 1996-12, Vol.53 (3), p.295-302
• Main Authors: Hines, Murray E., Cray, Carolyn, Elvinger, François, Altman, Norman H.
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; Anti-MIF-A3 antiserum; Antibodies; Bacteriology; Biological and medical sciences; Candida albicans; Cells, Cultured; Free Radical Scavengers - pharmacology; Fundamental and applied biological sciences. Psychology; Glycolipids - immunology; Glycolipids - isolation & purification; Glycolipids - pharmacology; Glycopeptides - immunology; Glycopeptides - isolation & purification; Glycopeptides - pharmacology; INHIBICION; INHIBITION; Lipopolysaccharides - pharmacology; MACROFAGOS; MACROPHAGE; Macrophage inhibition; Macrophage inhibitory factor-A3 (MIF-A3); MACROPHAGES; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - microbiology; Macrophages, Peritoneal - physiology; Mice; Mice, Inbred A; Mice, Inbred C3H; Mice, Inbred C57BL; Microbiology; Murine peritoneal macrophages; MYCOBACTERIUM AVIUM; Mycobacterium avium - chemistry; Mycobacterium avium serovar 2; Mycobacterium avium subsp. paratuberculosis; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; PERITOINE; PERITONEO; PERITONEUM; Phagocytosis - drug effects; Species Specificity; Tetradecanoylphorbol Acetate - pharmacology
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/S0378-1135(96)01220-5

Macrophage inhibitory factor-A3 (MIF-A3), a fraction derived from Mycobacterium avium serovar 2 inhibited candidacidal activity in macrophages from C57BL 6 , C57BL 10 , C3H HeJ and A J strains of mice. Inhibition of candidacidal activity was demonstrated at MIF-A3 concentrations ranging from 100–400 μg/ml in macrophages without additional stimulators (exception C3H HeJ macrophages) and in macrophages additionally stimulated with 200 U/ml interferon-γ, 100 ng/ml phorbol myristate acetate and 0.4 ng/ml E. coli lipopolysaccharide from all mouse strains tested. The decreased candidacidal effect produced by MIF-A3 was dose-dependent and appeared greatest in macrophages treated with phorbol myristate acetate and lipopolysaccharide. This effect was neutralized by the addition of goat anti-MIF-A3 antiserum. Macrophages from the Bcg s mouse strains ( C57BL 6 and C57BL 10 ) were more sensitive to the effect(s) of MIF-A3 than macrophages from the Bcg r mouse strains ( C3H HeJ and A J ).