Induction of xylanase and beta-xylosidase in Cellulomonas flavigena growing on different carbon sources

When Cellulomonas flavigena CDBB-531 was grown on glucose, xylose, glycerol, solka floc, sugarcane bagasse or xylan, xylanase activity was found only in the fermentation broth, while beta-xylosidase activity was always associated with the cells. Both enzymes were inducible, sugar-cane bagasse was th...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1996-11, Vol.46 (4), p.405-409
Main Authors: Perez-Avalos, O, Ponce-Noyola, T, Magana-Plaza, I, Torre, M. de la
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Sciences
Biotechnology
Cellulomonas flavigena
Enzyme engineering
Fermentation
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Production of selected enzymes
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Summary:When Cellulomonas flavigena CDBB-531 was grown on glucose, xylose, glycerol, solka floc, sugarcane bagasse or xylan, xylanase activity was found only in the fermentation broth, while beta-xylosidase activity was always associated with the cells. Both enzymes were inducible, sugar-cane bagasse was the best inducer, solka floc and avicel were moderately good, while xylan was poor. A synergistic effect on xylanase and beta-xylosidase synthesis was observed when cellulose and hemicellulose were used together as carbon sources. When this strain was grown on glucose, cellobiose, arabinose or xylose, only low levels of both enzymes were detected. These results indicate that xylanase and beta-xylosidase were carbon-source-repressed by readily metabolizable substrates. The effect of glycerol on enzymes that were already induced was studied. The addition of glycerol caused a significant decrease in the levels of xylanases, while beta-xylosidase activity remained unchanged.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00166237