High-frequency transformation of Staphylococcus aureus by electroporation

Plasmids carrying resistances for a variety of antibiotics were successfully transformed into Staphylococcus aureus by electrophoration. When frozen cell preparations were used, the procedure could be carried out in as little as 30 min. Frequencies as high as 7.6 x 10 super(5) transformants/ mu g of...

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Bibliographic Details
Published in:Current microbiology 1990-12, Vol.21 (6), p.373-376
Main Authors: KRAEMER, G. R, IANDOLO, J. J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
electroporation
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Summary:Plasmids carrying resistances for a variety of antibiotics were successfully transformed into Staphylococcus aureus by electrophoration. When frozen cell preparations were used, the procedure could be carried out in as little as 30 min. Frequencies as high as 7.6 x 10 super(5) transformants/ mu g of DNA were obtained with DNA isolated from both large-scale plasmid preparations and from minilysate preparations. A procedure was also developed for preparation and freezing (-70 degree C) of electrocompetent cells. These preparations have remained electrotransformable for 2 months without loss of activity.
ISSN:0343-8651
1432-0991
DOI:10.1007/BF02199440