Human bronchoepithelial cells in vitro as a tool for detection of genotoxic activity of airborne particulates

An experiment was conducted to determine the genotoxic activity of airborne particulates using bronchoepithelial cells as an in vitro target tissue. Airborne particulates were collected with a high volume sampler on glass fiber filters in the highly industrialized Rhine-Ruhr area from the city of Du...

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Bibliographic Details
Published in:Journal of aerosol science 1996-01, Vol.27, p.no. Sul 1-no. Sul 1
Main Authors: Hornberg, Claudia, Maciuleviciute, Lolita, Seemayer, N H
Format: Article
Language:English
Online Access:Get full text
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Summary:An experiment was conducted to determine the genotoxic activity of airborne particulates using bronchoepithelial cells as an in vitro target tissue. Airborne particulates were collected with a high volume sampler on glass fiber filters in the highly industrialized Rhine-Ruhr area from the city of Duisburg. Extracts of airborne particulates were added in various concentrations to the cell cultures in the presence of bromdeoxyuridine and demecolcine. After hypotonic treatment and fixation of cells, staining of the chromosomes was performed by fluorescent plus Giemsa technique. Induction of sister chromatid exchanges as a sensitive bioassay was used to evaluate the mutagenic activity of airborne particulate extract.
ISSN:0021-8502