Production of human growth hormone by protein-free cultivation of anchorage-dependent cells with porous cellulose carrier

• Published in: Journal of fermentation and bioengineering 1996-01, Vol.82 (5), p.469-474
• Main Authors: Nomura, Nakao, Wang, Pi-Chao, Saijo, Kaoru, Ohno, Tadao, Matsumura, Masatoshi
• Format: Article
• Language: English
• Subjects: Animal cells; ANIMAL GROWTH PROMOTERS; Biological and medical sciences; Biotechnology; CELL CULTURE; CELLULOSE; CELULOSA; CULTIVO DE CELULAS; CULTURE DE CELLULE; Establishment of new cell lines, improvement of cultural methods, mass cultures; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Health. Pharmaceutical industry; HORMONAS; HORMONE; HORMONES; Hormones. Growth factors; Industrial applications and implications. Economical aspects; Methods. Procedures. Technologies; porous cellulose carrier; Production of active biomolecules; PROMOTEUR DE CROISSANCE ANIMALE; PROMOTORES DEL CRECIMIENTO ANIMAL; protein-free; rotary column bioreactor; temperature-sensitive; Verots S3
• ISSN: 0922-338X
• DOI: 10.1016/S0922-338X(97)86985-1

Verots S3 cells derived from Vero-317 cells which can grow in biotin-containing MEM medium were successfully cultured in a protein-free medium with a porous cellulose carrier. The growth of Verots S3 cells without a carrier was inhibited because they easily aggregated under excessive shear stress, but the cells grew very quickly when cultured with the porous cellulose carrier. This improvement in cell growth was thought to be due to the protection from fluid shear stress afforded by the porous structure of the carrier. Production of human growth hormone (hGH) by repeated batch cultivation of Verots S3 cells was much better with than without the carrier. Cultivation with a temperature shift from 39 to 33°C gave hGH production up to 400% higher in a spinner culture with the cellulose carrier. For large-scale cultivation, temperature sensitive Verots S3 cells were cultivated with the porous cellulose carrier in a rotary column bioreactor and the perfusion culture results showed that the cell density inside the carrier increased up to 7.9 × 10 8 cells·ml-carrier −1 in 2 weeks. No concentration gradient of the cells in the rotary column was observed.