Excessive excretion of cyclic beta-(1,2)-glucan by Rhizobium trifolii TA-1

At 25 degrees C, the optimal temperature for growth of Rhizobium trifolii TA-1, extracellular and capsular polysaccharide (EPS and CPS) were the main carbohydrate products synthesized in mannitol-rich medium (10 g of mannitol and 1 g of glutamic acid per liter). In the same medium at 33 degrees C, E...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1990-07, Vol.56 (7), p.2080-2086
Main Authors: Breedveld, M.W. (Agricultural University, Wageningen, The Netherlands), Zevenhuizen, L.P.T.M, Zehnder, A.J.B
Format: Article
Language:English
Subjects:
beta-Glucans
Culture Media
EXCRECION
EXCRETION
GLUCANE
GLUCANO
Glucans - metabolism
MANNITOL
Mannitol - pharmacology
MEDIO DE CULTIVO
MILIEU DE CULTURE
Polysaccharides, Bacterial - biosynthesis
RHIZOBIUM
Rhizobium - drug effects
Rhizobium - growth & development
Rhizobium - metabolism
TEMPERATURA
TEMPERATURE
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Summary:At 25 degrees C, the optimal temperature for growth of Rhizobium trifolii TA-1, extracellular and capsular polysaccharide (EPS and CPS) were the main carbohydrate products synthesized in mannitol-rich medium (10 g of mannitol and 1 g of glutamic acid per liter). In the same medium at 33 degrees C, EPS and CPS production was inhibited, and up to 3.9 g of cyclic beta-(1,2)-glucan was produced during an incubation period of 20 days with a total biomass of 0.55 g of protein. In a medium containing 50 g of mannitol and 10 g of glutamic acid per liter, high cell densities (3.95 g of protein) were obtained at 25 degrees C. This biomass excreted 10.9 g of cyclic beta-(1,2)-glucan within 10 days. Concomitantly, 4.8 g of EPS was synthesized, while CPS production was strongly suppressed. The excreted cyclic beta-(1,2)-glucans were neutral and had degrees of polymerization ranging from 17 to 25, with a degree of polymerization of 19 as the major glucan cycle
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.56.7.2080-2086.1990