The CS6 colonization factor of human enterotoxigenic Escherichia coli contains two heterologous major subunits

Abstract The genes encoding the CS6 colonization factor were cloned from two human enterotoxigenic Escherichia coli strains of different serotypes. The DNA sequences from both clones were nearly identical and contained four open reading frames. Two of them have homology to genes encoding molecular c...

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Bibliographic Details
Published in:FEMS microbiology letters 1997-03, Vol.148 (1), p.35-42
Main Authors: Wolf, Marcia K, De Haan, Louise A.M, Cassels, Frederick J, Willshaw, Geraldine A, Warren, Richard, Boedeker, Edgar C, Gaastra, Wim
Format: Article
Language:English
Subjects:
Antigens, Bacterial - genetics
Antigens, Surface - genetics
Bacterial Proteins - genetics
Chaperones
Cloning, Molecular
Colonization
Colonization factor
Deoxyribonucleic acid
DNA
DNA, Bacterial - analysis
E coli
Enterotoxigenic Escherichia coli
Escherichia coli
Escherichia coli - classification
Escherichia coli - genetics
Escherichia coli Proteins
Gene Expression Regulation, Bacterial - genetics
Gene sequencing
Genes
Homology
Microbiology
Molecular Sequence Data
Mutagenesis, Site-Directed
Nucleotide sequence
Open reading frames
Operon - genetics
Operons
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Serotypes
Serotyping
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Summary:Abstract The genes encoding the CS6 colonization factor were cloned from two human enterotoxigenic Escherichia coli strains of different serotypes. The DNA sequences from both clones were nearly identical and contained four open reading frames. Two of them have homology to genes encoding molecular chaperones and ushers found in many other operons encoding colonization factors. The two remaining open reading frames encode two heterologous major subunit proteins which makes CS6 unique because other colonization factors have only one major subunit. Upstream and downstream of the CS6 operon the DNA sequences of the clones diverged abruptly.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1997.tb10263.x