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The CS6 colonization factor of human enterotoxigenic Escherichia coli contains two heterologous major subunits
Abstract The genes encoding the CS6 colonization factor were cloned from two human enterotoxigenic Escherichia coli strains of different serotypes. The DNA sequences from both clones were nearly identical and contained four open reading frames. Two of them have homology to genes encoding molecular c...
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| Published in: | FEMS microbiology letters 1997-03, Vol.148 (1), p.35-42 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c4385-84a640fc0c645dc0a103bdbd7ae3745bbc78ac1618147efbd2bfe93f6dbf2c9d3 |
| container_end_page | 42 |
| container_issue | 1 |
| container_start_page | 35 |
| container_title | FEMS microbiology letters |
| container_volume | 148 |
| creator | Wolf, Marcia K De Haan, Louise A.M Cassels, Frederick J Willshaw, Geraldine A Warren, Richard Boedeker, Edgar C Gaastra, Wim |
| description | Abstract
The genes encoding the CS6 colonization factor were cloned from two human enterotoxigenic Escherichia coli strains of different serotypes. The DNA sequences from both clones were nearly identical and contained four open reading frames. Two of them have homology to genes encoding molecular chaperones and ushers found in many other operons encoding colonization factors. The two remaining open reading frames encode two heterologous major subunit proteins which makes CS6 unique because other colonization factors have only one major subunit. Upstream and downstream of the CS6 operon the DNA sequences of the clones diverged abruptly. |
| doi_str_mv | 10.1111/j.1574-6968.1997.tb10263.x |
| format | article |
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The genes encoding the CS6 colonization factor were cloned from two human enterotoxigenic Escherichia coli strains of different serotypes. The DNA sequences from both clones were nearly identical and contained four open reading frames. Two of them have homology to genes encoding molecular chaperones and ushers found in many other operons encoding colonization factors. The two remaining open reading frames encode two heterologous major subunit proteins which makes CS6 unique because other colonization factors have only one major subunit. Upstream and downstream of the CS6 operon the DNA sequences of the clones diverged abruptly.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1997.tb10263.x</identifier><identifier>PMID: 9066107</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Antigens, Bacterial - genetics ; Antigens, Surface - genetics ; Bacterial Proteins - genetics ; Chaperones ; Cloning, Molecular ; Colonization ; Colonization factor ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - analysis ; E coli ; Enterotoxigenic Escherichia coli ; Escherichia coli ; Escherichia coli - classification ; Escherichia coli - genetics ; Escherichia coli Proteins ; Gene Expression Regulation, Bacterial - genetics ; Gene sequencing ; Genes ; Homology ; Microbiology ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Nucleotide sequence ; Open reading frames ; Operon - genetics ; Operons ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Serotypes ; Serotyping</subject><ispartof>FEMS microbiology letters, 1997-03, Vol.148 (1), p.35-42</ispartof><rights>1997 Federation of European Microbiological Societies. Published by Elsevier Science B.V. 1997</rights><rights>1997 Federation of European Microbiological Societies. Published by Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4385-84a640fc0c645dc0a103bdbd7ae3745bbc78ac1618147efbd2bfe93f6dbf2c9d3</citedby><cites>FETCH-LOGICAL-c4385-84a640fc0c645dc0a103bdbd7ae3745bbc78ac1618147efbd2bfe93f6dbf2c9d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9066107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolf, Marcia K</creatorcontrib><creatorcontrib>De Haan, Louise A.M</creatorcontrib><creatorcontrib>Cassels, Frederick J</creatorcontrib><creatorcontrib>Willshaw, Geraldine A</creatorcontrib><creatorcontrib>Warren, Richard</creatorcontrib><creatorcontrib>Boedeker, Edgar C</creatorcontrib><creatorcontrib>Gaastra, Wim</creatorcontrib><title>The CS6 colonization factor of human enterotoxigenic Escherichia coli contains two heterologous major subunits</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
The genes encoding the CS6 colonization factor were cloned from two human enterotoxigenic Escherichia coli strains of different serotypes. The DNA sequences from both clones were nearly identical and contained four open reading frames. Two of them have homology to genes encoding molecular chaperones and ushers found in many other operons encoding colonization factors. The two remaining open reading frames encode two heterologous major subunit proteins which makes CS6 unique because other colonization factors have only one major subunit. Upstream and downstream of the CS6 operon the DNA sequences of the clones diverged abruptly.</description><subject>Antigens, Bacterial - genetics</subject><subject>Antigens, Surface - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Chaperones</subject><subject>Cloning, Molecular</subject><subject>Colonization</subject><subject>Colonization factor</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - analysis</subject><subject>E coli</subject><subject>Enterotoxigenic Escherichia coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - classification</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli Proteins</subject><subject>Gene Expression Regulation, Bacterial - genetics</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Homology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Site-Directed</subject><subject>Nucleotide sequence</subject><subject>Open reading frames</subject><subject>Operon - genetics</subject><subject>Operons</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Serotypes</subject><subject>Serotyping</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqVUU2P1CAAJcbNOq7-BBOiibdWKC0UEw-bya6azGYPu54JUNjStDAWmp3110szkz0YPcgBDu-Dl_cAeI9RifP5NJS4YXVBOW1LzDkrk8KooqQ8vACbZ-gl2CDC2gIjzl6B1zEOCKG6QvQcnHNEKUZsA_x9b-D2jkIdxuDdL5lc8NBKncIMg4X9MkkPjU9mDikc3IPxTsOrqHszO907uQpdvnySzkeYHgPszcoew0NYIpzkkJ3iohbvUnwDzqwco3l7ei_Aj-ur--23Ynf79fv2clfomrRN0daS1shqpGnddBpJjIjqVMekIaxulNKslRpT3OKaGau6SlnDiaWdspXmHbkAH4---zn8XExMYnJRm3GU3uRUAjctJ7mETPzwB3EIy-xzNlERghlnrOaZ9fnI0nOIcTZW7Gc3yflJYCTWScQg1t7F2rtYJxGnScQhi9-dvljUZLpn6WmDjH854o9uNE__4Syub3akyfrmqA_L_h_q4m-5fgMyNq4w</recordid><startdate>199703</startdate><enddate>199703</enddate><creator>Wolf, Marcia K</creator><creator>De Haan, Louise A.M</creator><creator>Cassels, Frederick J</creator><creator>Willshaw, Geraldine A</creator><creator>Warren, Richard</creator><creator>Boedeker, Edgar C</creator><creator>Gaastra, Wim</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>199703</creationdate><title>The CS6 colonization factor of human enterotoxigenic Escherichia coli contains two heterologous major subunits</title><author>Wolf, Marcia K ; De Haan, Louise A.M ; Cassels, Frederick J ; Willshaw, Geraldine A ; Warren, Richard ; Boedeker, Edgar C ; Gaastra, Wim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4385-84a640fc0c645dc0a103bdbd7ae3745bbc78ac1618147efbd2bfe93f6dbf2c9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Antigens, Bacterial - genetics</topic><topic>Antigens, Surface - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Chaperones</topic><topic>Cloning, Molecular</topic><topic>Colonization</topic><topic>Colonization factor</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - analysis</topic><topic>E coli</topic><topic>Enterotoxigenic Escherichia coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - classification</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli Proteins</topic><topic>Gene Expression Regulation, Bacterial - genetics</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Homology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Site-Directed</topic><topic>Nucleotide sequence</topic><topic>Open reading frames</topic><topic>Operon - genetics</topic><topic>Operons</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Serotypes</topic><topic>Serotyping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolf, Marcia K</creatorcontrib><creatorcontrib>De Haan, Louise A.M</creatorcontrib><creatorcontrib>Cassels, Frederick J</creatorcontrib><creatorcontrib>Willshaw, Geraldine A</creatorcontrib><creatorcontrib>Warren, Richard</creatorcontrib><creatorcontrib>Boedeker, Edgar C</creatorcontrib><creatorcontrib>Gaastra, Wim</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolf, Marcia K</au><au>De Haan, Louise A.M</au><au>Cassels, Frederick J</au><au>Willshaw, Geraldine A</au><au>Warren, Richard</au><au>Boedeker, Edgar C</au><au>Gaastra, Wim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The CS6 colonization factor of human enterotoxigenic Escherichia coli contains two heterologous major subunits</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1997-03</date><risdate>1997</risdate><volume>148</volume><issue>1</issue><spage>35</spage><epage>42</epage><pages>35-42</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract
The genes encoding the CS6 colonization factor were cloned from two human enterotoxigenic Escherichia coli strains of different serotypes. The DNA sequences from both clones were nearly identical and contained four open reading frames. Two of them have homology to genes encoding molecular chaperones and ushers found in many other operons encoding colonization factors. The two remaining open reading frames encode two heterologous major subunit proteins which makes CS6 unique because other colonization factors have only one major subunit. Upstream and downstream of the CS6 operon the DNA sequences of the clones diverged abruptly.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9066107</pmid><doi>10.1111/j.1574-6968.1997.tb10263.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1997-03, Vol.148 (1), p.35-42 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15893906 |
| source | Oxford Journals Online |
| subjects | Antigens, Bacterial - genetics Antigens, Surface - genetics Bacterial Proteins - genetics Chaperones Cloning, Molecular Colonization Colonization factor Deoxyribonucleic acid DNA DNA, Bacterial - analysis E coli Enterotoxigenic Escherichia coli Escherichia coli Escherichia coli - classification Escherichia coli - genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial - genetics Gene sequencing Genes Homology Microbiology Molecular Sequence Data Mutagenesis, Site-Directed Nucleotide sequence Open reading frames Operon - genetics Operons Sequence Analysis, DNA Sequence Homology, Amino Acid Serotypes Serotyping |
| title | The CS6 colonization factor of human enterotoxigenic Escherichia coli contains two heterologous major subunits |
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