Construction of GFP vectors for use in Gram-negative bacteria other than Escherichia coli

Abstract A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn...

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Bibliographic Details
Published in:FEMS microbiology letters 1996-11, Vol.145 (1), p.87-94
Main Authors: Matthysse, Ann G., Stretton, Serina, Dandie, Catherine, McClure, Nicholas C., Goodman, Amanda E.
Format: Article
Language:English
Subjects:
Bacteria
Bacterial promoter probe
Cloning, Molecular
Coliforms
Deoxyribonucleic acid
DNA
DNA Transposable Elements - genetics
DNA, Bacterial - genetics
E coli
Escherichia coli
Escherichia coli - genetics
Genes, Reporter - genetics
Genetic Vectors
Gram-negative bacteria
Gram-Negative Bacteria - genetics
Green fluorescent protein
Green Fluorescent Proteins
Host range
Luminescent Proteins - genetics
Microbiology
Mutagenesis - genetics
Plant pathogenic bacteria
Plasmids
Plasmids - genetics
Polymerase Chain Reaction
Restriction Mapping
Strains (organisms)
Transposon
Vectors
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Summary:Abstract A set of vectors containing a mutated gfp gene was constructed for use with Gram-negative bacteria other than Escherichia coli. These constructs were: pTn3gfp for making random promoter probe gfp insertions into cloned DNA in E. coli for subsequent introduction into host strains; pUTmini-Tn5gfp for making random promoter probe gfp insertions directly into host strains; p519gfp and p519ngfp, broad host range mob+ plasmids containing gfp from a lac and an npt2 promoter, respectively.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1996.tb08561.x