Laboratory studies identify a colloidal groundwater tracer: implications for bioremediation

We have identified and tested a new colloidal tracer for use in hazardous waste site characterization and other environmental applications. The tracer is primarily composed of the dead ice nucleating active (INA) bacterium Pseudomonas syringae. Assay conditions are simple and based on the observatio...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1997-03, Vol.148 (2), p.131-135
Main Authors: Strong-Gunderson, J.M, Palumbo, A.V
Format: Article
Language:English
Subjects:
Bioremediation
Colloids
Contaminants
Detection limits
environmental degradation
Fracture flow
Freezing
Groundwater
Groundwater flow
Hazardous waste sites
Hazardous wastes
Ice nucleating active bacterium
Microbiology
Microsphere bead
pollution
Pseudomonas syringae
Sodium chloride
Waste disposal sites
waste management
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have identified and tested a new colloidal tracer for use in hazardous waste site characterization and other environmental applications. The tracer is primarily composed of the dead ice nucleating active (INA) bacterium Pseudomonas syringae. Assay conditions are simple and based on the observation of the freezing behavior of 10 microliter solution volumes. Under specific assay conditions (-5 to -7 degrees C) these drops freeze only if the tracer is present. The results are available within 3 min of sample collection thus, providing near real-time results. The tracer detection limits are in the range of ng/l, it is stable over a pH range of 2-11, and maintains its high activity in the presence of a variety of contaminant compounds. In a simple column experiment this colloid tracer eluted just prior to NaCl with little or no tailing.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1997.tb10278.x