Priming of rabbit alveolar macrophages for enhanced oxidative responses by herpes simplex virus type 2 infection

• Published in: Journal of leukocyte biology 1991-05, Vol.49 (5), p.442-448
• Main Authors: Giridhar, G., Hayakawa, H., Kucera, L.S., Myrvik, Q.N.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cell Transformation, Viral; Cells, Cultured; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; herpes simplex virus 2; Immunobiology; Kinetics; latex; Luminescent Measurements; Luminol; Macrophages - drug effects; Macrophages - immunology; Macrophages - physiology; Male; Monocytes, macrophages; Mycobacterium bovis - immunology; Myeloid cells: ontogeny, maturation, markers, receptors; Oxygen Consumption; phorbol ester; Rabbits; respiratory burst; Simplexvirus - genetics; Tetradecanoylphorbol Acetate - pharmacology
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.49.5.442

The effect of herpes simplex virus type 2 (HSV‐2) infection on the oxidative response in infant and adult rabbit alveolar macrophages (AM) was studied using either phorbol myristate acetate (0.5 μg PMA/ml) or latex (250 μg/ml) as eliciting agents in a chemiluminescence (CL) assay. Results indicated that uninfected infant AM responded to a latex‐elicited but not PMA‐elicited CL response. HSV‐2 infection (moi=1.0) of infant AM for 2 hr at 37° did not alter the PMA or latex‐elicited CL responses. In contrast, uninfected adult AM exhibited a markedly increased CL response when elicited with either PMA or latex. HSV‐2 infection (moi=1) of adult AM for 2 hr further increased both PMA‐ and latex‐elicited CL responses. Increasing the moi to 10 inhibited both PMA‐ and latex‐elicited CL responses. Incubation of uninfected control and HSV‐2 infected adult AM for 18 hr at 37° resulted in spontaneous priming of the cells for increased CL responses. In the absence of PMA HSV‐2 alone failed to elicit a CL response in adult AM. Infection with heat‐inactivated HSV‐2 (moi=1.0 before heat inactivation) did not prime adult AM for enhanced CL responses. AM from BCG immunized adult rabbit produced a considerably higher level CL response that nonimmunized AM; however, HSV‐2 infection of these cells did not further enhance the response. In summary, these data indicate that adult AM but not infant AM can be primed by active HSV‐2 infection for an increased CL response elicited by either PMA or latex.