Loading…

Generation of calcium oscillations in fibroblasts by positive feedback between calcium and IP sub(3)

In this study, Ca super(2+) was found to stimulate its own release in REF52 fibroblasts primed by mitogens plus depolarization. However, unlike Ca super(2+) release in muscle and nerve cells, this amplification was insensitive to caffeine or ryanodine and required hormone receptor occupancy and func...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1991-01, Vol.251 (4989), p.75-78
Main Authors: Harootunian, A T, Kao, Joseph PY, Paranjape, S, Tsien, R Y
Format: Article
Language:English
Subjects:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, Ca super(2+) was found to stimulate its own release in REF52 fibroblasts primed by mitogens plus depolarization. However, unlike Ca super(2+) release in muscle and nerve cells, this amplification was insensitive to caffeine or ryanodine and required hormone receptor occupancy and functional IP sub(3) receptors. Oscillations in (Ca super(2+)) sub(i) were accompanied by oscillations in IP sub(3) concentration but did not require functional protein kinase C. Therefore, the dominant feedback mechanism in this cell type appears to be Ca super(2+) stimulation of phospholipase C once this enzyme has been activated by hormone receptors.
ISSN:0036-8075