Genetic relationship of Mediterranean mandarins (Citrus deliciosa tenore) using RAPD markers

Random amplified polymorphic DNA (RAPD) analysis was carried out to evaluate polymorphism and genetic similarity between 39 Mediterranean mandarin genotypes. One hundred eleven amplification products were identified using 21 random primers. An average of 2.2 RAPD markers was obtained for each primer...

Full description

Saved in:
Bibliographic Details
Published in:Euphytica 1996, Vol.92 (3), p.321-326
Main Authors: MACHADO, M. A, COLETTA, H. D, TARGON, M. L. P. N, POMPEU, J. JR
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biotechnology
Classical genetics, quantitative genetics, hybrids
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetic resources, diversity
Genetics and breeding of economic plants
Genetics of eukaryotes. Biological and molecular evolution
Plant material
Pteridophyta, spermatophyta
Taxonomy
Vegetals
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Random amplified polymorphic DNA (RAPD) analysis was carried out to evaluate polymorphism and genetic similarity between 39 Mediterranean mandarin genotypes. One hundred eleven amplification products were identified using 21 random primers. An average of 2.2 RAPD markers was obtained for each primer, corresponding to 42% of the amplification products. Genotype-specific RAPD markers were also found, mainly in known hybrids. UPGMA cluster analysis revealed the low level of genetic variation between accessions of Mediterranean mandarins, whereas their hybrids with other Citrus species showed greater genetic dissimilarity. Twenty accessions yielded very similar patterns, suggesting either that they could be a single clone, or that the technique was not able to detect genomic variation. However, for the other specimens genetic polymorphism can easily be detected by RAPD, although the genetic variation between accessions was quite low. The large number of hybrids and the low polymorphism between accessions support the hypothesis that Mediterranean mandarins are all true hybrid of Common mandarins (Citrus reticulata Blanco).
ISSN:0014-2336
1573-5060