Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli

The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xyl...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1990-10, Vol.34 (1), p.71-76
Main Authors: Bhalerao, J, Patki, A.H, Bhave, M, Khurana, I, Deobagkar, D.N
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Cellulomonas
cloning
enzyme activity
Escherichia coli
Fundamental and applied biological sciences. Psychology
gene expression
gene transfer
genes
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Molecular cloning
O-glycoside hydrolases
plasmids
structural genes
xylan endo-1,3- beta -xylosidase
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Summary:The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00170926