An in vivo micronucleus assay for detecting the clastogenic effect in rat kidney cells

A micronucleus assay in vivo has been developed that is based on the use of freshly isolated kidney cells from mononephrectomized rats. In this validation study, a statistically significant increase in the frequency of micronucleated cells was detected in rats given i.p. a single dose of four kidney...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 1997-04, Vol.390 (1), p.51-57
Main Authors: Robbiano, Luigi, Mereto, Eugenio, Migliazzi Morando, Andrea, Pastore, Patrizia, Brambilla, Giovanni
Format: Article
Language:English
Subjects:
Clastogenic activity
in vivo
Kidney carcinogen
Micronucleus assay
N-ethyl- N-hydroxyethylnitrosamine
N-nitroso- N-methylurea
N-Nitrosodiethylamine
N-nitrosodimethylamine
Rat kidney cell
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Summary:A micronucleus assay in vivo has been developed that is based on the use of freshly isolated kidney cells from mononephrectomized rats. In this validation study, a statistically significant increase in the frequency of micronucleated cells was detected in rats given i.p. a single dose of four kidney carcinogens, N-nitrosodimethylamine, N-nitrosodiethylamine, N-ethyl- N-hydroxyethylnitrosamine and N-nitroso- N-methylurea. The clastogenic effect was more marked when the same dose was injected for 3 successive days. As compared to controls, treated rats displayed a reduction in the frequency of binucleated cells, presumably due to a toxicity-induced inhibition of cellular proliferation. The proposed method should be suitable for the detection of the clastogenic effect of procarcinogens biotransformed into reactive species in the kidney.
ISSN:1383-5718
1879-3592
DOI:10.1016/S0165-1218(96)00165-6